Methods and compositions for modifying shade avoidance in plants
AssigneePairwise Plants Services, Inc.
InventorBrian Charles Wilding Crawford
Life of this patent
Estimating the term…
Abstract
This invention relates to compositions and methods for modifying an Elongated Hypocotyl5 (HY5) transcription factor in plants to suppress the shade avoidance response. The invention further relates to plants and plant parts produced using the methods and compositions of the invention.
Claims33
A plant or plant part thereof comprising at least one mutation in an endogenous Elongated Hypocotyl5 (HY5) gene encoding a HY5 transcription factor, wherein the endogenous HY5 gene:
- (a) comprises a sequence having at least 80% sequence identity to the nucleotide sequence of SEQ ID NO:72 or SEQ ID NO:73;
- (b) comprises a region having at least 80% sequence identity to any one of the nucleotide sequences of SEQ ID NOs:75-105;
- (c) encodes a polypeptide comprising a sequence having at least 80% sequence identity to the amino acid sequence of SEQ ID NO:74;
- and/or
- (d) encodes a region having at least 80% sequence identity to the amino acid sequence of SEQ ID NO:106.
(canceled)
(canceled)
(canceled)
12-15. (canceled)
18-19. (canceled)
21-57. (canceled)
A method for producing a plant or part thereof comprising at least one cell having a mutation in an endogenous Elongated Hypocotyl5 (HY5) gene, the method comprising contacting a target site within the endogenous HY5 gene in the plant or plant part with a nuclease comprising a cleavage domain and a nucleic acid binding domain, wherein the nucleic acid binding domain of the nuclease binds to a target site within the endogenous HY5 gene, wherein the endogenous HY5 gene:
- (a) comprises a sequence having at least 80% sequence identity to the nucleotide sequence of SEQ ID NO:72 or SEQ ID NO:73;
- (b) comprises a region having at least 80% sequence identity to any one of the nucleotide sequences of SEQ ID NOs:75-105;
- (c) encodes a polypeptide comprising a sequence having at least 80% sequence identity to the amino acid sequence of SEQ ID NO:74;
- and/or
- (d) encodes a region having at least 80% sequence identity to the amino acid sequence of SEQ ID NO:106, thereby producing a plant or part thereof comprising at least one cell having a mutation in the endogenous HY5 gene.
(canceled)
61-63. (canceled)
65-68. (canceled)
72-77. (canceled)
A guide nucleic acid that binds to a target site within an endogenous HY5 gene, the endogenous HY5 gene comprising a sequence having at least 80% sequence identity to the nucleotide sequence of SEQ ID NO:72 or SEQ ID NO:73;
- comprising a region having at least 80% sequence identity to any one of the nucleotide sequences of SEQ ID NOs:75-105;
- encoding a polypeptide comprising a sequence having at least 80% sequence identity to the amino acid sequence of SEQ ID NO:74;
- and/or encoding a region having at least 80% sequence identity to the amino acid sequence of SEQ ID NO:106, optionally wherein the guide nucleic acid comprises a spacer having the nucleotide sequence of any one of SEQ ID NOs:107-113.
79-88. (canceled)
A nucleic acid encoding a mutated Elongated Hypocotyl5 (HY5) transcription factor comprising a sequence having at least 90% sequence identity to any one of SEQ ID NOs:114, 116, 118, 119, 121, 123, 125, 127, 129, 131, 133, 135, 137, or 139 and/or encoding a mutated HY5 polypeptide having at least 90% sequence identity to any one of SEQ ID NOs:115, 117, 120, 122, 124, 126, 128, 130, 132, 134, 136, or 138.
90-91. (canceled)
93-104. (canceled)
Dependencies are inferred from the claim text.