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AssigneeMonsanto Technology LLC
InventorsEdward J. CARGILL, Linda A. RYMARQUIS, Thomas L. SLEWINSKI, Michelle VALENTINE
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The present disclosure provides compositions and methods for altering auxin accumulation in corn or other cereal plants. Methods and compositions are also provided for altering the expression of genes related to auxin efflux through editing of a Brachytic2 (br2) gene to introduce an antisense sequence or segment or a deletion or premature stop codon into the gene. Modified plant cells and plants having a dominant or semi-dominant allele reducing the expression or activity of a br2 gene product are further provided comprising improved characteristics, such as reduced plant height and increased lodging resistance, but without off-types in the plant.
A modified corn plant, or plant part thereof, comprising a mutant allele of an endogenous Brachytic2 (br2) locus, wherein the mutant allele comprises a DNA segment inserted into the endogenous br2 locus, wherein the DNA segment encodes an antisense RNA sequence that is at least 85% complementary to at least 20 consecutive nucleotides of one or more of SEQ ID NOs:
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A method for producing a mutant allele of an endogenous Brachytic2 (br2) locus, the method comprising:
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A method for generating a corn plant comprising:
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Dependencies are inferred from the claim text.