Method of producing globin polypeptide recombinantly and meat substitute food product
AssigneePlaneat Foods Pte. Ltd.
InventorsTuck Seng WONG, Kang Lan TEE, Margit LANGWALLNER, Madhavan NALLANI, Christoph LANGWALLNER
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Abstract
The present invention refers to a method of producing globin polypeptide recombinantly and a method of producing globin polypeptide with a cell-free translation system. Further, the present invention provides a food product, preferably a meat substitute food product. The present invention is also directed to a vector and a system for recombinant globin polypeptide production as well as to a cell comprising a recombinant expression vector or one or more recombinant nucleic acid molecule(s).
Claims16
Method of producing globin polypeptide recombinantly, comprising:
- transforming a host cell with an expression vector comprising
- one or more nucleic acid sequence(s) encoding said globin polypeptide, wherein said globin polypeptide is/are selected from the group consisting of a leghemoglobin from a Vigna plant, a leghemoglobin from a lupin, a myoglobin from bovine, a bacterial hemoglobin having at least 70% sequence identity with the bacterial hemoglobin of Vitreoscilla stercoraria, and any combination thereof,
- wherein the host cell is selected from yeast or bacteria;
- culturing said host cell under conditions effective for the expression of said one or more nucleic acid sequence(s);
- expression of said one or more nucleic acid sequence(s), and
- recovering said globin polypeptide from the obtained culture.
Method of producing globin polypeptide with a cell-free translation system, comprising:
- Providing one or more nucleic acid sequence(s) encoding the globin polypeptide, wherein the globin polypeptide is/are selected from the group consisting of a leghemoglobin from a Vigna plant, a leghemoglobin from a lupin, a myoglobin from bovine, a bacterial hemoglobin having at least 70% sequence identity with the bacterial hemoglobin of Vitreoscilla stercoraria, and any combination thereof,
- translating the one or more nucleic acid sequence(s) with the cell-free translation system, and
- recovering the globin polypeptide from the cell-free translation system.
A food product, preferably a meat substitute food product, comprising:
- one or more globin protein(s), wherein said one or more globin protein(s) is/are selected from the group consisting of a leghemoglobin from a Vigna plant, a leghemoglobin from a lupin, a myoglobin from bovine, a bacterial hemoglobin having at least 70% sequence identity with the bacterial hemoglobin of Vitreoscilla stercoraria, and any combination thereof;
- one or more fibres, preferably one or more fibres from a plant, more preferably one or more fibres from a legume or a grain,
- one or more carbohydrates, preferably one or more carbohydrates from a plant, more preferably one or more carbohydrates from a legume or a grain,
- one or more fats, preferably one or more fats from non-animals,
- one or more micronutrients,
- one or more other proteins than the one or more globin protein(s), preferably one or more protein(s) from a plant, more preferably one or more protein(s) from a legume or a grain,
- one or more flavors, yeast extracts, hydrolized vegetable proteins, herbs and/or seasoning, preferably plant flavors and/or plant seasoning,
- optionally one or more egg albumin,
- optionally one or more hydrocolloid,
- optionally one or more mycoprotein, and
- optionally one or more cell based protein.
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Dependencies are inferred from the claim text.