Application of helz2 in drugs for preventing and treating tumors
AssigneeJiangsu Concord Biotechnology Co., Ltd
InventorsHuabiao CHEN, Tao LI
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Abstract
The present disclosure provides a vector containing a target sequence that targets and interferes with Helicase with zinc finger 2 (Helz2), the target sequence comprising one or more of the following sequences: SEQ ID No. 5: 5′-GCTATCAAGTCTGTCACTACT-3′; SEQ ID No. 6: 5′-GCACGATGCTGTATGGCTTTG-3′; SEQ ID No. 7: 5′-GGGCCTCATTGACACTCAAAG-3′.
Claims16
A vector containing a target sequence that targets and interferes with Helicase with zinc finger 2 (Helz2), the target sequence comprising one or more of the following sequences:
- SEQ ID No. 5:
- 5′-GCTATCAAGTCTGTCACTACT-3′;
- SEQ ID No. 6:
- 5′-GCACGATGCTGTATGGCTTTG-3′;
- SEQ ID No. 7:
- 5′-GGGCCTCATTGACACTCAAAG-3′.
A method for establishing an Helz2 knockout mouse model, comprising:
- performing Helz2 knockout on fertilized mouse eggs by using CRISPR/Cas9 technique with an sgRNA sequence;
- and
- obtaining a systemic Helz2 knockout homozygous mouse model through microinjection and breeding of the fertilized mouse eggs.
An sgRNA sequence for establishing an Helz2 knockout animal model, comprising one or more of the following sequences:
- SEQ ID No. 1:
- 5′-GCCCCAGAGTTACCAGATGGAGG-3′;
- SEQ ID No. 2:
- 5′-CCTACACCCGACAGAGGTGTAGG-3′;
- SEQ ID No. 3:
- 5′-AGCAGTGACAGTCTTATGGGTGG-3′;
- SEQ ID No. 4:
- 5′-GGCATACAGAGGATTGCCACAGG-3′.
A drug for treating or preventing a tumor, comprising:
- a product targeting an Helz2 gene or targeting an expression of the Helz2 gene.
Dependencies are inferred from the claim text.