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US 2023/0416744 A1Published application
Methods and compositions for increased double stranded rna production
AssigneeRnaissance AG LLC
InventorsJohn L. KILLMER, Patrick D. McLAUGHLIN, Juan Pedro Humberto ARHANCET
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Abstract
The invention provides methods and compositions for improved production of large quantities of unencapsidated double strand RNA (dsRNA) in vivo. The disclosed methods and compositions, comprising co-expression of genes encoding orotate phosporibosyl transferase, bacteriophage coat protein and dsRNA produce a significant improvement over current in vivo methods of producing unencapsidated dsRNA.
Claims20
A microbial cell comprising
- (1) a coat protein gene encoding a capsid protein, wherein the capsid protein is the capsid protein of bacteriophage MS2, the capsid protein of bacteriophage Qβ, or a fragment thereof, and
- (2) a gene encoding a heterologous dsRNA molecule comprising a self-complementary stretch of sequence separated by noncomplementary sequence such that upon hybridization of the complementary sequences a stem-loop structure is formed, and
- wherein the stem-loop structure formed has a length exceeding the interior diameter of an MS2 capsid.
Dependencies are inferred from the claim text.