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US 2023/0416724 A1Published application
Lysis Buffers Comprising Cyanate or Thiocyanate and a Detergent
OwnerNathan P. Hoverter, Lewis A. Marshall +3inventors
InventorsNathan P. Hoverter, Lewis A. Marshall, Klint A. Rose, Debannita Datta, Douglas Fadrosh
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Abstract
The present disclosure provides cell lysis buffers and method of use. Cell lysis buffers may comprise an aqueous solution comprising a buffering agent, a detergent, and cyanate or thiocyanate. The cell lysis buffers may be free or substantially free of urea and/or carbonic acid. Such lysis buffers may be used to lyse cells, e.g., in tissues. The cell lysis buffers may be suitable for use with isotachophoresis.
Claims26
A composition comprising:
- a) a buffer in aqueous solution;
- b) a detergent;
- and
- c) about 5 mM to about 100 mM cyanate or thiocyanate;
- wherein a relative concentration of the cyanate or the thiocyanate to urea in the composition is at least about 1:800, at least about 1:600, at least about 1:400, at least about 1:200, at least about 1:10, at least about 1:1, at least about 100:1, at least about 10,000:1, or at least about 1,000,000:1.
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Dependencies are inferred from the claim text.