Process for the production of a technical enzyme composition with low viscosity produced by a filamentous fungus
AssigneeClariant Produkte (Deutschland) GmbH
InventorsChristian GAMAUF, Jörg CLAREN
Life of this patent
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Abstract
The present invention relates to a process for the production of a technical enzyme composition with low viscosity produced by a genetically modified filamentous fungus, a genetically modified filamentous fungus suitable for production of the technical enzyme composition, the use of such a genetically modified filamentous fungus for the production of the technical enzyme composition with low viscosity and a technical enzyme composition with low viscosity produced by such a process.
Claims18
Process for production of a whole broth enzyme composition, comprising the following steps:
- (a) providing a fermentation medium, originating from hydrolysis of lignocellulosic biomass, with a glucose content of from 5 to 450 g/L, a xylose content of from 2 to 300 g/L, a density of from 1 to 2 kg/L and a dry matter content of from 10 to 75 wt.-%;
- (b) adding at least one filamentous fungus cell wherein SEQ ID NO:1 has been disrupted;
- (c) mixing the fermentation medium and the at least one filamentous fungus cell for a time period of from 1 minute to 10 days at a temperature of from 20 to 35° C.;
- and
- (d) obtaining a whole broth enzyme composition;
- wherein the filamentous fungus cell is selected from the species Trichoderma reesei.
Filamentous fungus cell wherein SEQ ID NO:1 has been disrupted, wherein the filamentous fungus cell is selected from the species Trichoderma reesei.
(canceled)
Dependencies are inferred from the claim text.