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US 2023/0416710 A1Published application
Engineered and chimeric nucleases
AssigneeMetagenomi, Inc.
InventorsBrian C. THOMAS, Christopher BROWN, Cristina BUTTERFIELD, Jyun-Liang LIN, Alan BROOKS, Morayma M. TEMOCHE-DIAZ, Greg COST, Rebecca LAMOTHE
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Abstract
Disclosed herein are engineered nucleases and nuclease systems, including chimeric nucleases and chimeric nuclease systems. Engineered and chimeric nucleases disclosed herein include nucleic acid guided nuclease. Additionally disclosed herein are methods of generating engineered nucleases and methods of using the same.
Claims22
A fusion endonuclease comprising:
- (a) an N-terminal sequence comprising at least part of a RuvC domain, a REC domain, or an HNH domain of an endonuclease having at least 80% sequence identity to SEQ ID NO:
- 696 or a variant thereof;
- and
- (b) a C-terminal sequence comprising WED, TOPO, or CTD domains of an endonuclease having at least 80% sequence identity to SEQ ID NO:
- 706 or 708 or variants thereof, wherein said N-terminal sequence and said C-terminal sequence do not naturally occur together in a same reading frame.
2 more elements
A fusion endonuclease comprising an engineered amino acid sequence having at least 80% sequence identity to any one of SEQ ID NOs:
- 10 or 12, or a variant thereof.
Dependencies are inferred from the claim text.