Methods and Materials for Producing Polyols and Electron Rich Compounds
AssigneeThe Trustees of Princeton University
InventorsYi-Fan Xu, Joshua D. Rabinowitz, Fabien Letisse, Sarah Johnson
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Abstract
Methods and materials for producing polyols are provided comprising recombinant microorganisms expressing a Pyp1 polyol phosphatase. Also provided herein are methods and materials for producing electron rich compounds in recombinant microorganisms lacking the DET1 and/or PHO13 genes.
Claims21
A recombinant S. cerevisiae cell comprising a polynucleotide encoding Pyp1, ortholog thereof, or a variant of Pyp1 at least 70% identical to the amino acid sequence of SEQ ID NO:
- 1, wherein the polynucleotide is operably linked to a heterologous promoter.
A recombinant microorganism comprising a polynucleotide encoding Pyp1, ortholog thereof, or a variant of Pyp1 at least 70% identical to the amino acid sequence of SEQ ID NO:
- 1.
A method of producing a polyol comprising:
- (a) culturing a recombinant microorganism comprising a polynucleotide encoding Pyp1, ortholog thereof, or a variant of Pyp1 at least 70% identical to the amino acid sequence of SEQ ID NO:
- 1 under conditions effective to express Pyp1, the ortholog thereof, or the variant of Pyp1;
- and optionally
- (b) separating the polyol from the culture.
(canceled)
(canceled)
A recombinant microorganism comprising a mutation or deletion of the DET1 gene or ortholog thereof and a mutation or deletion of the PHO13 gene or ortholog thereof.
15-17. (canceled)
(canceled)
A method of producing an electron rich compound comprising:
- (a) culturing a Saccharomyces cerevisiae cell comprising a deletion or mutation of the DET1 gene and/or PHO13 gene, under conditions that promote over-expression of one or both of (i) a polynucleotide encoding an enzyme with sugar phosphate dehydrogenase activity and a gene encoding a polyol phosphatase or (ii) and engineered biosynthetic pathway for the electron rich compounds;
- and optionally
- (b) separating the electron rich compound from the culture.
23-27. (canceled)
Dependencies are inferred from the claim text.