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US 2023/0416702 A1Published application
Stable synthetic phytase variants
AssigneeEw Nutrition GmbH
InventorsAndreas MICHELS, Andreas SCHEIDIG, Thomas HORN, Alexander METTEN
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Abstract
The present invention relates to a synthetic phytase which is a shuffling product comprising biobricks of at least two reference phytases of Obesumbacterium and/or Hafnia.
Claims18
A synthetic phytase which is a shuffling product comprising biobricks of at least two reference phytases of Obesumbacterium and/or Hafnia
- which phytase comprises one of
- a) an amino acid sequence according to SEQ ID NO 3 (“shuffled sequence”)
- b) the amino acid sequence according to option a), with the proviso that the sequence has at least 1 amino acid substitutions relative to SEQ ID NO 3 as specified in a), while maintaining its phytase activity,
- c) the amino acid sequence according to option a), with the proviso that the sequence has a sequence identity of ≥90% relative to SEQ ID NO 3 as specified in a), while maintaining its phytase activity.
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Dependencies are inferred from the claim text.