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US 2023/0416693 A1Published application
Improved washing methods for affinity chromatography
OwnerPeter S. GAGNONinventor
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Abstract
A method for removal of contaminants in a sample of AAV comprising the steps of loading the sample to an affinity chromatography column, washing the column after the sample is loaded with a first buffer comprising a compound having at least three positive charges or at least three negative charges employing a second buffer having a salt concentration corresponding to a concentration of at least 0.25 M NaCl for displacing the compound wherein neither the compound nor the salt is present in a concentration sufficient to elute the AAV.
Claims22
A method for removal of contaminants in a sample of AAV comprising the steps of
- loading the sample to an affinity chromatography column;
- washing the column after the sample is loaded with a first buffer comprising a compound having at least three positive charges or at least three negative charges;
- and
- employing a second buffer having a salt concentration corresponding to a concentration of at least 0.25 M NaCl for displacing the compound,
- wherein neither the compound nor the salt is present in a concentration sufficient to elute the AAV.
2 more elements
Dependencies are inferred from the claim text.