Method for manufacturing cell mass

A method for manufacturing a cell mass adherently cultured on a cell culture substrate in which the cell culture substrate comprises the following two regions (A) and (B), and the method comprising the following steps (1) to (3):

• (A) an island-shaped region having a cell proliferation property and an area of 0.001 to 1 mm2;
• and
• (B) a region adjacent to the region (A) and having no cell proliferation properties,
• (1) a step of preparing a cell suspension comprising a mesenchymal cell and an ectoderm-derived cell;
• (2) a step of bringing the cell suspension prepared in the step (1) into contact with the cell culture substrate, and randomly adhering the mesenchymal cell and the ectoderm-derived cell to the region (A), and in which, on the contact surface between the cell suspension and the cell culture substrate, a total number of cells of the mesenchymal cell and the ectoderm-derived cell is 100 to 100,000 cells per unit area;
• and
• (3) a step of culturing the cell adhered to the region (A) and forming a cell mass comprising the mesenchymal cell and the ectoderm-derived cell.