Chemical culture system and use thereof
AssigneeIregene Therapeutics Ltd
InventorsJUN WEI, Meng CAI
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Abstract
A chemical culture system and use thereof. The chemical culture system comprises a basic culture medium and a small molecule compound, and forms a serum-free culture system having a clear chemical composition. The culture medium does not use substitute serum B27 and GS21 which contain animal-derived components and are widely used currently, but uses a pure chemical molecule activation signal pathway, and uses a non-animal-derived growth factor to replace an animal-derived growth factor, and thus the composition is clear, the potential risks caused by the existence of serum and animal-derived components are avoided, and the clinical prospect of nerve cell transplantation is expanded.
Claims18
A chemical culture system characterized by comprising a basic culture medium and additives;
- the additives comprise a small molecular compound, the small molecular compound is:
- piperidinol oxide and luteolin;
- or, at least one of piperidinol oxide, luteolin at least one of D(+)-galactose, recombinant human transferrin;
- the dosage of the piperidinol oxide is 10 μM-200 μM;
- the dosage of the luteolin is 5 μM-150 μM;
- the dosage of the D(+)-galactose is 5-25 μg/mL;
- the dosage of the recombinant human transferrin is 50-200 ng/mL.
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Dependencies are inferred from the claim text.