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US 2023/0416678 A1Published application
Cell culture medium
AssigneeAuckland Uniservices Limited
InventorsPeter Roderick Dunbar, Vaughan John Feisst, Inken Dorothea Kelch
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Abstract
The present invention relates to a growth medium, which does not include cholera toxin, for culturing cells, such as epithelial cells, in particular keratinocytes, use of the medium to grow cells, (for example epithelial cells, such as keratinocytes) and kits comprising the medium. Also provided is a method of generating human full thickness skin, fully human epidermis or fully human dermis involving culturing keratinocytes and feeder cells in said cell culture medium.
Claims21
(canceled)
A cholera toxin-free cell culture medium consisting of components:
- DMEM, Ham's F12, serum, one or more antibiotic(s) and/or antimycotic(s), keratinocyte growth factor (KGF) and a ROCK inhibitor.
Dependencies are inferred from the claim text.