Composition And Method For Stabilizing And Maintaining The Viability Of Hardy Microorganisms
AssigneeDna Genotek Inc.
InventorsH. Chaim Birnboim, Cassandra Kelly-Cirino, Bitapi Ray, Jacques Oscar Everard Niles, Olle Maarten de Bruin
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Abstract
The present application is to provide a composition and method for stabilizing and maintaining the viability of hardy microorganisms from sample collection to downstream analysis. In particular, there is a method for preserving viable hardy bacteria, such as Mycobacteria, Bacillus anthracis, or Clostridium difficile, in a biological sample, comprising contacting the biological sample with a stabilization composition, wherein the stabilization composition comprises a chelating agent, a denaturing, a salt and has a p H between about 6 and about 11.
Claims22
A method for stabilizing the microbiome within a biological sample comprising contacting the biological sample with a stabilization composition, wherein the stabilization composition comprises a chelating agent, a denaturing agent, a salt and has a pH between 6 and 11.
A method for characterization of bacterial nucleic acid from viable hardy bacteria in a biological sample comprising said viable hardy bacteria, comprising:
- contacting the biological sample with a stabilization composition, wherein the stabilization composition comprises a chelating agent, a denaturing agent, a salt and has a pH between 6 and 11;
- and
- amplifying the nucleic acid in the sample, wherein the level of amplified nucleic acid remains substantially unchanged if the amplification step occurs immediately after collection, or later.
A composition comprising:
- a chelating agent;
- a denaturing agent;
- and
- viable hardy microorganisms.
Dependencies are inferred from the claim text.