Three-Dimensional Thin Film Structure Having Microparticles Enclosed Therein And Method For Manufacturing Same

A biological tissue-like structure comprising:

• a polymer film having a plurality of layers, wherein a cell is encapsulated in an internal space of said structure, and each layer of the polymer film having the plurality of layers has mechanical strengths different from each other;
• a second cell existing outside the three-dimensional structure;
• wherein the cell encapsulated in the structure forms a structure extending to an outside of the structure, and
• an intercellular interaction is able to occur between the cell encapsulated in the structure and the second cell existing outside the structure.

A method for producing a three-dimensional structure encapsulating a microparticle,

• the method comprising the steps of:
• (a) forming a polymer film having a plurality of layers
• (b) floating the microparticle over a surface of the polymer film having the plurality of layers and
• (c) generating a stress distribution in a thickness direction in the polymer film having the plurality of layers to form a three-dimensional structure in a self-assembling manner in the polymer film having the plurality of layers.

A laminate comprising:

• a substrate;
• a sacrificial layer laminated on the substrate;
• and
• a polymer film having a plurality of layers laminated on the sacrificial layer,
• wherein each layer of the polymer film having the plurality of layers is composed of a polymer material that may generate a stress distribution in a thickness direction in the polymer film when the polymer film is released from the substrate by decomposing the sacrificial layer.