Cassette for sterility testing

A method comprising:

• assembling a cell culturing device comprising a lid having an optically clear window, a fluid distribution channel, a sample injection port fluidically connected to the fluid distribution channel, a base that comprises a porous media pad, one or more outlet ports arranged around a perimeter of the media pad;
• mating a first connector of a tube set to the sample injection port;
• introducing sample media through the tube set into the sample injection port;
• placing the cell culturing device into an incubator;
• after a predefined interval, automatically retrieving the cell culturing device from the incubator;
• automatically sending the cell culturing device through an imaging station;
• causing the cell culturing device to be subjected to excitation light of predetermined wavelengths by the imaging station to cause microbial growth present on the membrane to exhibit fluorescence;
• and
• capturing an image of the fluorescence.