Methods Of Identifying And Evaluating Cat Allergy Gene Signatures In A Subject By Determining A Stratified Score Based On Gene Expression

A method of identifying a subject as suitable for the treatment or prevention of asthma exacerbation induced by a cat allergen with at least one antibody to the cat allergen, the method comprising:

• a) generating or having generated a first transcriptome for the subject before exposure to at least one cat allergen;
• b) exposing the subject to the at least one cat allergen;
• c) treating the subject with at least one antibody to the cat allergen;
• d) generating or having generated a second transcriptome for the subject after treatment with at least one antibody to the cat allergen;
• and
• e) screening the second transcriptome against the first transcriptome to generate a cat allergen exposure/treatment transcriptome;
• wherein the subject is suitable for the treatment or prevention of asthma exacerbation induced by a cat allergen with at least one antibody to the cat allergen when the cat allergen exposure/treatment transcriptome comprises:
• a downregulation of at least any five of the following genes:
• BC007880, BC080587, BX537909, NGS-17, LOC100862671, AX747215, P2RX1, SDS, S1PR4, RPPH1, ARPC4-TTLL3, and SERPING1;
• and
• an upregulation of at least any twenty-five of the following genes:
• C6orf165, ARHGEF33, LOC157381, ARHGEF26-AS1, IQUB, WDR78, CNTD1, TEX21P, REEP1, RBM24, TMEM212, TEX9, DIO1, ECT2L, GCNT4, FSD1L, MAMDC2, LOC100499484-C90RF174, ADH6, LOC653501, DNAH7, DCDC2, KLHL32, PTGES3L, ADAM22, AKAP6, TEX26, DPY19L2, ZBBX, TXLNB, ANKFN1, CHDC2, CRISPLD1, WDR96, FAM216B, AK057689, TSPAN19, DTHD1, PROS1, DNAH5, AX747851, MYLK, AK090700, SRD5A2, ANGPTL5, TMEM232, CXorf22, AK097702, AK310634, C1orf141, BICC1, FAM86B2, AX747150, PCDHB1, CAPS2, PTPN20B, TCP11X2, GIPC2, GRM5, LOC100130451, C12orf63, FLJ00322, MTTP, MROH9, SLC16A12, AK307870, MYCT1, AK094325, AX747187, FGF14, PHOSPHO2-KLHL23, CD200R1L, AK126334, PALM2-KAP2, and LY75-CD302.

A method of identifying a subject as suitable for the treatment or prevention of asthma exacerbation induced by a cat allergen with at least one antibody to the cat allergen, the method comprising:

• a) exposing the subject to at least one cat allergen;
• b) generating or having generated a third transcriptome for the subject after exposure to at least one cat allergen;
• c) treating the subject with at least one antibody to the cat allergen;
• d) exposing the subject to at least one cat allergen;
• e) generating or having generated a fourth transcriptome for the subject after exposing the subject to at least one cat allergen in step d);
• and
• e) screening the fourth transcriptome against the third transcriptome to generate a cat allergen exposure/treatment/exposure transcriptome;
• wherein the subject is suitable for the treatment or prevention of asthma exacerbation induced by a cat allergen with at least one antibody to the cat allergen when the cat allergen exposure/treatment/exposure transcriptome comprises:
• a downregulation of at least any ten of the following genes:
• LOC100132062, CCL8, AK128563, BC048201, LOC100133669, IL17D, RGS13, DKFZp434J0226, PMF1-BGLAP, LOC643802, AK123177, SPP1, BC041030, AK001975, THBS4, BC047364, CNGB3, FAM27C, SRMS, CXCL13, COL22A1, BC064137, AK055853, OCLM, PSORS1C2, AX746492, KCNA7, EFCAB3, NCAM1, PRR5-ARHGAP8, CNDP1, MSI1, BX648826, GFRA3, DACT1, AX748314, CNN1, BC107108, PCP2, LRRC63, SNAP25-AS1, ESYT3, HIST1H1D, AK129879, CASQ2, SLC16A10, CRISP3, KIAA0931, DQ588114, AX747628, AK022787, GFRA1, LIN7A, OXER1, LILRA5, AX748175, AK125412, LHFPL5, PTPLA, AX748417, AF119915, EMX2, PCDHGC4, SEPT12, BPIFB4, CILP, ADAMTS1, BHMT2, LINC00937, AX747864, TLDC2, BMP6, MMP9, MAP1LC3C, MSRB3, AK095583, LOC729444, DQ599872_2, MAGOH2, BC041484, TTYH1, PLA2G4E, PDE1B, CCR3, C22orf26, NAT2, AK125288, DPEP3, EDA2R, PYHIN1, CYSLTR2, GPR83, AX746564_2, SMIM1, AK311167, 7SK 25, LOC100288142, PAI1, ANKS4B, LMO3, AK097351, AK054921, PATE2, AK057519, KIAA0087, AK310030, CPXM2, RUNX1T1, LOC100506801, TMEM31, ZNF724P, AK311374, GNGT2, GRID1, PTCD1, AB209185, PPP1R27, FUT7, NXPH2, NECABI, BC039386, S100A5, SFTPD, KU-MEL-3, SALL1, BC070322, MYH6, AF086102, CLUL1, APOBEC2, RPL23P8, COL19A1, LRFN2, RAB3A, ANO3, RPS2P32, FOLR2, AK055332, FBN3, MYOZ3, NCRUPAR, AX748230, CCDC110, SLC13A1, LOC100270746, EPOR, TMEM252, LRFN1, CES1P2, MAL, ADH1A, ILDR2, CA10, SCHIP1, SND1-IT1, BC042024, NHSL2, KLRF2, PIEZO2, AL390170, BC021693, FAM198B, TIPARP-AS1, CD22, OLFM3, GNB3, BC042046, BC041007, DRD1, PGLYRP4, IGSF9B, KIF5A, FKSG63, AK123947, OR4F29, NKD2, PCDHA6, FOSL1, LAMB2P1, GBP1P1, AX746775, FRMD1, PCDHB9, AK301549, ALB, Metazoa_SRP_39, LOC100288911, ENTPD1-AS1, AX747405, FAM180A, MI0003602, ZNF891, SPATA31D5P, LSMEM1, PMS2L14, AK093534, TEX40, AK097590, SERPINB7, BC047540, KRT73, BC049825, TMPRSS6, TMEM200B, MAGEA2, BC047723, BMP2, HSD3B2, AIPL1, KCNA2, BC029473, SCN4A, CCDC116, GGN, KCNJ8, MI0000285, DDX4, LOC283177, MMP7, REM2, CYP17A1, NINJ2, LOC100506060, AX746750, ADAMTSL5, GPR143, WASH6P, CD2, SPDYE6, U4_5, and MKRN3;
• and
• an upregulation of at least any twenty of the following genes:
• ITGA9, RAB40AL, APLN, DCSTAMP, COL8A1, VRTN, SSMEM1, ALDH1L1-AS1, HNF1A, TOB2P1, INO80B, Metazoa_SRP_17, COL4A4, LINC00112, UGT2B28, VAX2, GAD1, CAPNS2, TUSC5, RBM46, LOC728739, LGSN, DQ574505, HYAL4, LOC100505474, TCRBV13S6A2T, C3orf70, UNC5D, TRAV20, CDKN3, ZNF774, RBM11, SHISA3, C9orf170, RBP5, NPAS1, CDY2A, STBD1, LOC441081, BC019672, CST9, P2RY4, CNBD1, DL490887, PCDHGA10, ZNF382, LDLRAD2, SLAMF9, CCNA2, AWAT2, AB586698, SLC25A21-AS1, MT1IP, OCA2, SFTA1P, AK057259, ATP1A10S, GCSAML-AS1, LOC100130557, NXNL2, HSF2BP, CYP4Z2P, FLJ41941, SNORA66, CD1A, AK301968, ROR2, LOC339290, ITIH1, AK300656, QRFPR, TCP11, SLC35F1, ASCL1, TCRBV9S1A1T, TRPM2, DCC, SIRPD, KRTAP5-2, LOC100133050, MB21D2, POLN, CARD9, ZNF556, LOC148709, MEP1A, STAU2-AS1, BSN, DQ658414, SLC2A14, SPESP1, BLOC1S5, F2RL3, AHSG, LOC100505841, IQGAP3, AK127124, FAM182B, BC030152, TEX21P, PABPC1P2, UMODL1, TCRBV3S1, FMO9P, BC033983, KRTAP5-10, RASAL2-AS1, AJ606331, SH2D6, DSG1, LEMD1, NKAIN2, CR933665, BX648502, LOC286297, LOC647859, BC053679, VWCE, JN120857, ALPPL2, ALX4, Metazoa_SRP_66, WEE2, SPARC, SPTB, SLC22A13, GPR37, POU6F2, F12, PDGFRA, TPPP2, RPL13AP20, KNCN, FLJ30838, AIFM3, BLOC1S1-RDH5, ZNF177, RAB9B, ZNF815P, TRAV12-1, LOC339047, BC070061, SLC2A5, BC046483, ATOH7, UTS2B, LOC100289388, MCF2L2, COL9A1, CXorf57, BC017910, SUMO1P3, MATN1-AS1, IGLL5, DQ577420, CCDC73, FAM95B1, LOC100289092, PNMA2, AK125437, SLC7A5P2, ZNF367, LOC100131060, C11orf53, MRVI1-AS1, RUSC1-AS1, LOC51326, DSCR9, TRGC2, NPSR1-AS1, ABCB4, AX746969, CRB2, ESM1, ADAMTS15, GPR25, CDH17, NIM1, FLJ38723, LOC100129138, LIN7B, SLC47A2, IL36G, GOLGA8T, WFDC1, FOXD2-AS1, OR7D2, GPR63, FOXD4L1, LOC100507537, BC036361, BC070106, BEND4, CYS1, FLJ23584, GRIP2, CKLF-CMTM1, SPINK6, SARDH, HS3ST3B1, LOC643723, DJ031142, SMAD5-S1, AJ420489, PDE2A, EIF5AL1, UCN, SNPH, ANO7, SPC25, AX747264, SNORA6, SEC14L4, HLA-DPB2, C2orf27A, AX747125, SSR4P1, TFAP2B, BC045163, KIF18B, LOC644145, RFPL1S, IL9R, SNORA52, DPRXP4, LOC285768, CHKB-AS1, BCL2L2-PABPN1, STXBP5L, AK309988, DLEU7, SLFNL1-AS1, GABRR1, C10orf55, LOC100131655, NR5A2, OLFM2, DL492607, ADAM12, TDRD9, FAM74A4, BC062769, SLC30A4, LOC339874, LOC100128531, AK097190, BC080653, PEG3, RBFADN, FAM64A, CR627206, ETV2, GPSM1, PKNOX2, AK309255, PI16, SLC4A10, TUBB4A, AKR1C4, C17orf67, AX746484, SLC7A9, LOC643355, LOC645166, SLC28A1, FAM225B, ZNF663, GLB1L3, AK055272, ASGR2, ZCCHC18, LINC00312, BMPER, SYT2, CAMK2A, GRK7, HEATR4, BC023651, AK095633, CSPG4, SNORA81, U1_10, BC065373, AK056432, BC040572, RAB40A, BC040208, HABP2, MGC16142, PEBP4, CCDC154, LOC440934, AK128837, BVES, IGSF1, AX746944, AB075492, MYH16, RFTN2, LOC100130015, TLE6, AX746627, MUC3B, CABC1, LOC285972, BC024248, COL5A3, NSFP1, ANKRD55, TRA@, PRSS30P, ZNF385D, AK098783, CORT, TLR9, KCND2, AK095546, FAM35BP, ATP5J2-PTCD1, RAD51L3-RFFL, and LOC727751.