Homodimeric and heterodimeric proteins comprising butyrophilin
AssigneeShattuck Labs, Inc.
InventorsTaylor SCHREIBER, George FROMM, Suresh DE SILVA
Life of this patent
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Abstract
The present disclosure relates, inter alia, to compositions and methods, including heterodimeric proteins and chimeric proteins comprising portions of butyrophilin family of proteins that find use in the treatment of disease, such as immunotherapies for cancer and autoimmunity.
Claims118
A heterodimeric protein comprising an alpha chain and a beta chain,
- wherein the alpha chain comprises:
- (a) a first domain comprising a BTN2A1 protein, or a fragment thereof;
- (b) a second domain comprising a targeting domain that specifically binds to CD19;
- and
- (c) a linker that adjoins the first and second domains;
- and
- wherein the beta chain comprises:
- (a) a first domain comprising a BTN3A1 protein, or a fragment thereof;
- (b) a second domain comprising a targeting domain that specifically binds to CD19;
- and
- (c) a linker that adjoins the first and second domains.
The tetrameric chimeric protein as depicted in FIG. 11, optionally comprising a polypeptide having an amino acid sequence that has at least about 95% identity with an amino acid sequence selected from SEQ ID NOs:
- 43, 44 and 56-70.
A chimeric protein of a general structure of:
- N terminus-(a)-(b)-(c)-C terminus,
- wherein:
- (a) is the first domain comprising the general structure of (a1)-SL-(a2), wherein
- (a1) is an extracellular domain (ECD) of a butyrophilin family protein, or a fragment thereof,
- (a2) is an extracellular domain (ECD) of a butyrophilin family protein, or a fragment thereof, and SL is a second linker adjoins (a1) and (a2) comprising a flexible amino acid sequence of about 4 to about 50 amino acids length, and
- (c) is a second domain comprising a targeting domain, the targeting domain being selected from (i) an antibody, antibody-like molecule, or antigen binding fragment thereof, and (ii) an extracellular domain of a membrane protein, and
- (b) is linker that adjoins the first and second domains, wherein the a linker comprises at least one cysteine residue capable of forming a disulfide bond.
Dependencies are inferred from the claim text.