High-purity ß-nicotinamide mononucleotide (nmn) and method for producing same
AssigneeMirailab Bioscience Inc.
InventorsTsunemaru TANAKA, Megumi TANAKA, Ryuji HIRAMATSU, Daizo SHIMAMURA
Life of this patent
Estimating the term…
Abstract
The present invention addresses the problem of a providing NMN having high purity and a low impurity content. The present invention is a high-purity β-nicotinamide mononucleotide (NMN) which has a purity of 99.0 mass % or higher and in which the amounts of C11H15NO9P, C10H16N5O10P2, C9H15N3O8P, C9H15N3O7P, C9H16N4O8P, C10H15N5O7P, and C21H27N6O15P2 contained as impurities are equal to or less than a detection limit.
Claims4
High-purity s-nicotinamide mononucleotide (NMN) having a purity of 99.0% by mass or more and having contents of C11H15NO9P, C10H16N5O10P2, C9H15N3O8P, C9H15N3O7P, C9H16N4O8P, C10H16N5O7P, and C21H27N6O15P2 as impurities equal to or less than a detection limit.
A method for producing high-purity β-nicotinamide mononucleotide (NMN), comprising the steps of:
- (I) reacting AMP nucleosidase, ribose-phosphate diphosphokinase, adenosine monophosphate (AMP), adenosine triphosphate (ATP), and ribose-5-phosphate (R5P);
- (II) reacting nicotinamide phosphoribosyltransferase (Nampt), a reaction product of the step (I), and nicotinamide (NAM);
- (III) purifying a reaction product of the step (II) with an ion-exchange column;
- and
- (IV) applying a purification product of the step (III) to a dynamic axial compression column (DAC column).
Dependencies are inferred from the claim text.