Method for manufacturing a biological analysis chip and biological analysis chip

Method of manufacturing a biological sample analysis chip, wherein the method comprises:

• a—two support matrices are provided, each formed in a solid support material, each having a lower surface and an upper surface and in each of which at least one through hole has been formed extending between said lower and upper surfaces, among which a lower support matrix and an upper support matrix are defined;
• b—at least two different sheets of solid and porous analysis material are provided, each sheet having a lower surface and an upper surface,
• c—the lower support matrix, the different sheets of analysis material and the upper support matrix are superimposed in that order so:
• that the upper surface of the lower support matrix faces the lower surface of the upper support matrix,
• that the at least one through hole of the lower support matrix is facing the at least one through hole of the upper support matrix,
• that the different sheets of analysis material are intercalated between the lower and upper support matrices,
• that the at least two sheets of analysis material are juxtaposed or intercalated;
• d—a mechanical assembly of the two support matrices and the sheet of analysis material is carried out during which a pressing force in a direction normal to the lower and upper surfaces of the support matrices is exerted so as to bring the support matrices closer to one another.