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US 2023/0414690 A1Published application
Full-Spectrum Plant-Derived Extracts Enriched In Total Phenols Including Monomers, Dimers, Trimers, Oligomers And Polymers, Method Of Manufacturing Same And Uses Thereof
AssigneePhenolics, LLC
InventorsMichael S. Tempesta, F. Joseph Daugherty
Life of this patent
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Abstract
Methods for preparing compositions highly enriched in phenolic compounds from plant materials that naturally contain phenolic compounds such as anthocyanins and proanthocyanidins are enriched for a wide spectrum of phenolic compounds and consist of phenolic compounds including monomers, dimers, trimers, oligomers and polymers >3000 amu and over 35% concentrated total phenols. The compositions of the present disclosure which include phenolic compounds including monomers, dimers, trimers, oligomers and polymers >3000 amu and over 35% concentrated total phenols optionally include trisodium citrate and/or cyclodextrin.
Claims54
An enriched, full-spectrum, polyphenolic extract derived from plant material, comprising:
- a) phenolic monomers, dimers and trimers;
- b) phenolic oligomers;
- and
- c) phenolic polymers;
- wherein the polyphenolic extract contains >35% by weight total phenols;
- wherein a phenolic compound in the polyphenolic extract is selected from the group consisting of catechins, epicatechins, proanthocyanidins, gallocatechins, galloepicatechins, anthocyanins, glycosides, acylglycosides, polyacylglycosides, anthocyanidins, phenolic acids of C6-C1 class, benzoic and hydroxybenzoic acids, C6-C3 class of acids, and cinnamic, chlorogenic, and related acids;
- and
- wherein at least 10% by weight of the polyphenolic extract are phenolic polymers.
5 more elements
A method of manufacturing an enriched, full-spectrum, polyphenolic extract derived from plant material, comprising:
- a) extracting and filtering juice from the plant material to obtain a raw extract;
- b) diluting the raw extract with acidified water to a juice strength extract;
- c) passing the juice strength extract through a PS-DVD crosslinked porous gel packed column to cause phenolic constituents in the juice strength extract to adhere to the column and non-phenolic contaminants to pass through the gel-packed column;
- d) washing the phenolic constituents adhering to the column with at least 3 to 20 column volumes of acidified water to remove sugars and organic acids to remove non-phenolic contaminants adsorbed/embedding in the phenolic constituents;
- e) eluting and collecting the phenolic constituents adhering to the gel column with lower alcohol/acidified water in the range of 50-90% to create a semi-purified polyphenolic extract;
- f) concentrating the semi-purified polyphenolic extract by removing ethanol and water therefrom and diluting the concentrated product with acidified water to an approximate volume of the juice strength extract to create a semi-purified eluent;
- g) loading the semi-purified eluent onto a second PS-DVD crosslinked porous gel packed column so as to cause phenolic constituents in the juice strength extract to adhere to the column and additional non-phenolic contaminants to pass through the gel-packed column;
- h) washing the phenolic constituents adhering to the column with at least 3 to 20 column volumes of acidified water to remove additional sugars, organic acids and non-phenolic contaminants adsorbed/embedding in the phenolic constituents;
- i) eluting the adhered polyphenols from the gel column with acidified alcohols containing 1-3 carbons to and collecting the eluent into an enriched polyphenolic eluent;
- and
- j) concentrating the enriched polyphenolic eluent removing liquid therefrom to obtain an enriched, full-spectrum, polyphenolic extract containing >35% total phenols and at least 10% by weight of the polyphenolic extract are >2.5 kDalton.
8 more elements
A method of manufacturing an enriched, full-spectrum, polyphenolic extract derived from plant material, comprising:
- a) extracting and filtering juice from the plant material to obtain a raw extract;
- b) diluting the raw extract with acidified water to a juice strength extract;
- c) passing the juice strength extract through a mixed resin PS-DVD crosslinked porous gel packed column to cause phenolic constituents in the juice strength extract to adhere to the column and non-phenolic contaminants to pass through the gel-packed column;
- d) washing the phenolic constituents adhering to the column with at least 3 to 20 column volumes of acidified water to remove sugars and organic acids to remove non-phenolic contaminants adsorbed/embedding in the phenolic constituents;
- e) eluting and collecting the phenolic constituents adhering to the gel column with lower alcohol/acidified water in 50-90% to create a semi-purified polyphenolic extract;
- f) concentrating the semi-purified polyphenolic extract by removing ethanol and water therefrom and diluting the concentrated product with acidified water to an approximate volume of the juice strength extract to create a semi-purified eluent;
- g) eluting the adhered polyphenols from the gel column with acidified alcohols containing 1-3 carbons to and collecting the eluent into an enriched polyphenolic eluent;
- and
- h) concentrating the enriched polyphenolic eluent removing liquid therefrom to obtain an enriched, full-spectrum, polyphenolic extract containing >35% total phenols and at least 10% by weight of the polyphenolic extract are >2.5 kDalton.
6 more elements
A method of manufacturing an enriched, full-spectrum, polyphenolic extract derived from plant material, comprising:
- a) extracting and filtering juice from the plant material to obtain a raw extract;
- b) diluting the raw extract with acidified water to a juice strength extract;
- c) passing the juice strength extract through individual PS-DVD crosslinked porous gel packed column to cause phenolic constituents in the juice strength extract to adhere to the column and non-phenolic contaminants to pass through the gel-packed column;
- d) washing the phenolic constituents adhering to the column with at least 3 to 25 column volumes of acidified water to remove sugars and organic acids to remove non-phenolic contaminants adsorbed/embedding in the phenolic constituents;
- e) eluting and collecting the phenolic constituents adhering to the gel column with lower alcohol/acidified water in 50-90% to create a semi-purified polyphenolic extract;
- f) concentrating the semi-purified polyphenolic extract by removing ethanol and water therefrom and diluting the concentrated product with acidified water to an approximate volume of the juice strength extract to create a semi-purified eluent;
- g) eluting the adhered polyphenols from the gel column with acidified alcohols containing 1-3 carbons to and collecting the eluent into an enriched polyphenolic eluent;
- and
- h) concentrating the enriched polyphenolic eluent removing liquid therefrom to obtain an enriched, full-spectrum, polyphenolic extract containing >35% total phenols and at least 10% by weight of the polyphenolic extract are >2.5 kDalton.
6 more elements
A method of use for enhancing uptake of phenolic components across the blood brain barrier in a subject, comprising administering to an individual a dose of:
- at least one phenolic compound;
- and
- a compound selected from the group consisting of a citrate and pharmaceutically acceptable salts thereof.
Dependencies are inferred from the claim text.