Methods and compositions for stem cell transplantation
AssigneeAngiocrine Bioscience, Inc.
InventorsMichael Daniel Ginsberg, Daniel Joseph Nolan, Claude Geoffrey Davis, Paul William Finnegan
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Abstract
The present disclosure relates to the field of hematopoietic stem or progenitor cell transplantation. More specifically, methods, compositions and kits for improving expansion and engraftment of hematopoietic stem or progenitor cells by co-culturing and co-administering with endothelial cells are provided. The methods, compositions and kits are useful for treating various disorders relating to deficiencies in hematopoiesis caused by disease or myeloablative treatments.
Claims66
A method of stem cell transplantation in a subject in need thereof comprising:
- (a) expanding hematopoietic stem or progenitor cells (HSPCs) by contacting the HSPCs with endothelial cells (ECs) at an initial EC/HSPC ratio for a first time period under conditions allowing for expansion of the HSPCs, to produce an expanded cell population comprising HSPCs and ECs at an expanded HSPC/EC ratio;
- and
- (b) transfusing the expanded cell population obtained from step (a) into the subject;
- wherein a continuous interaction is maintained between the HSPCs and the ECs throughout the expansion and the transfusion steps.
Dependencies are inferred from the claim text.