Stem cell-produced microvesicles for treating tendon pathologies
AssigneeAlbert Einstein College of Medicine
InventorHui B. Sun
Life of this patent
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Abstract
Microvesicles produced by stem cells grown on a silk scaffold for enhancing stem cell self-renewal/proliferation and promoting tenogenesis in damaged tendons, and their use in tendon wound repair and tendinopathy treatment. Also provided are compositions containing the microvesicles and devices for obtaining them.
Claims20
1-35. (canceled)
A method of treating a damaged tendon in a first mammalian subject comprising:
- obtaining, or having obtained, an amount of mesenchymal stem cells from a subject of the same species as the first mammalian subject;
- culturing the mesenchymal stem cells on a scaffold so as to produce vesicles;
- and
- administering to the first mammalian subject an amount of the vesicles effective to treat a damaged tendon,
- or
- a method of differentiating stem cells into a tendon lineage by physical means comprising:
- obtaining, or having obtained, an amount of mesenchymal stem cells from a mammalian subject;
- culturing the mesenchymal stem cells on a scaffold comprising a 3D scaffold comprising a plurality of fibers substantially aligned in a parallel manner along their longitudinal axes and held under tension in a longitudinal direction of the fibers, so as to induce elongation of the mesenchymal stem cells and tendon lineage differentiation.
A system comprising a 3D scaffold comprising a plurality of fibers substantially aligned in a parallel manner along their longitudinal axes and held under tension in a longitudinal direction of the fibers, and mesenchymal stem cells obtained from a mammalian subject present on the 3D scaffold, and vesicles derived from the mesenchymal stem cells.
A composition comprising a sterile pharmaceutically acceptable carrier and an amount of vesicles derived from a supernatant of mesenchymal stem cells cultured on a 3D scaffold comprising a plurality of fibers substantially aligned in a parallel manner along their longitudinal axes and held under tension in a longitudinal direction of the fibers.
Dependencies are inferred from the claim text.