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AssigneeThe Trustees of Columbia Universit In the City of New York
InventorsMegan Sykes, Robert J. Hawley, Kazuhiko Yamada, David H. Sachs
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Provided herein are recombinant miniature swine expressing human CD47 in a tissue specific fashion. Also provided are kidneys isolated from a miniature swine, wherein the glomeruli of the kidney express human CD47 at a level higher than the level of human CD47 expression in the tubules of the kidney. Furthermore, provided herein are methods of transplanting such swine kidneys with glomeruli-specific expression of human CD47 from recombinant miniature swine into human recipients. In certain aspects, provided herein are methods of transplantation comprising transplanting hematopoietic stem cells expressing human CD47 from a first donor animal (e.g., a miniature swine) and a kidney expressing human CD47 in the glomeruli from a second donor animal (e g., a miniature swine) to a recipient (e.g., a human recipient).
A method for preventing or reducing the severity of proteinuria in a kidney transplant recipient, wherein the method comprises:
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A kidney isolated from a miniature swine, wherein the glomeruli of the kidney express human CD47 at a level higher than the level of human CD47 expression in the tubules of the kidney, optionally wherein the glomeruli of the kidney express human CD47 at a level 2 times to 10 times higher than the level of human CD47 expression in the tubules of the kidney.
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A method of transplanting a kidney from a miniature swine into a human recipient, wherein the method comprises:
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A xenograft from a non-human species, wherein the xenograft comprises:
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Dependencies are inferred from the claim text.