Novel methods
OwnerPayal ARORA, Chider CHENinventors
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Abstract
The invention relates to methods of treatment and screening natural compounds that can be used to further proliferate orally derived stem cells (e.g., periodontal ligament stem cells, dental pulp stem cells, or stem cells from the apical papilla or gingiva-derived mesenchymal stem cells (GMSC)).
Claims20
A method of treatment of gingiva and/or periodontal ligament tissue to increase dental osteogenesis, mineralization or soft tissue regeneration in the oral cavity, wherein the method comprises administering an effective amount of one or more natural ERK activating compounds to the oral cavity of a patient in need thereof,
- wherein the patient has existing endogenous stem cells that are present in the oral cavity, and;
- wherein the natural ERK activating compound is administered to the endogenous stem cells in the oral cavity of the patient.
A method of selecting natural ERK activating compounds that cause the proliferation of endogenous oral stem cells wherein the method comprises:
- (1) identifying natural ERK activating test compounds by observing increasing in mineralization relative to and a negative control, and/or gene expression of osteogenic biomarkers relative to a positive control and a negative control;
- (2) isolating periodontal ligament stem cell cells and/or or gingiva-derived mesenchymal stem cells from an oral cavity,
- (3) identifying periodontal ligament stem cells and/or or gingiva-derived mesenchymal stem cells,
- (4) contacting and screening periodontal ligament stem cells and/or or gingiva-derived mesenchymal stem cells with identified natural ERK activating test compounds;
- (5) determining whether a test compound results in the proliferation of periodontal ligament stem cells and/or or gingiva-derived mesenchymal stem cells;
- and
- (6) selecting a test compound for further development on the basis of whether it increases proliferation of periodontal ligament stem cells and/or or gingiva-derived mesenchymal stem cells relative to a standard control.
Dependencies are inferred from the claim text.