High-purity steviol glycosides
AssigneePureCircle Sdn Bhd
InventorsAvetik MARKOSYAN, Indra PRAKASH, Cyrille JARRIN, Aurélien BADIE, Robert TER HALLE, Cynthia CURRAN, Daniel AURIOL
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Abstract
Methods of preparing highly purified steviol glycosides, particularly rebaudiosides A, D and Mare described. The methods include utilizing recombinant microorganisms for converting various staring compositions to target steviol glycosides. In addition, novel steviol glycosides reb D2, reb M2, and reb I are disclosed, as are methods of preparing the same. The highly purified rebaudiosides are useful as non-caloric sweetener in edible and chewable compositions such as any beverages, confectioneries, bakery products, cookies, and chewing gums.
Claims12
-33. (canceled)
A method for producing a highly purified target steviol glycoside composition, comprising the steps of:
- a. providing a starting composition comprising steviol glycosides;
- b. providing UDP-glycosyltransferases and optionally UDP-glucose recycling enzymes;
- c. contacting the UDP-glycosyltransferases with a medium comprising the starting composition to produce a composition comprising a target steviol glycoside;
- and
- d. separating the target steviol glycoside from the medium to provide a highly purified target steviol glycoside composition.
The highly purified target steviol glycoside composition of claim 7, wherein the target steviol glycoside is selected from reb D, reb M and reb I.
Dependencies are inferred from the claim text.