Methods of stable vitrification
AssigneeUpkara, Inc.
InventorsPravansu Mohanty, Mary Retzlaff, Laura Bronsat, Yolanda Taverner, Zhouran Wang, Animesh Koneru
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Abstract
Disclosed are methods for non-cryogenic vitrification of biological materials that include the steps of providing a biological sample and vitrification medium on a capillary substrate network within a desiccation chamber and providing both a heat energy and a lowered atmospheric pressure to provide for rapid vitrification without the vitrification medium or biological sample exhibiting cryogenic temperature or boiling as a result of lowered atmospheric pressure.
Claims76
A process for vitrification of one or more biological materials above cryogenic temperature, the process comprising:
- a) overlaying a vitrification mixture comprising a biological sample and a vitrification medium on a substrate comprising a capillary network, said substrate in a desiccation chamber;
- b) lowering the atmospheric pressure within the desiccation chamber;
- c) providing a heat energy from the surface to the vitrification mixture, wherein the heat energy is sufficient to prevent the vitrification mixture from experiencing a freezing condition;
- and
- d) desiccating the vitrification mixture by capillary action until the vitrification mixture enters a glassy state.
Dependencies are inferred from the claim text.