Triticale cultivar 343cms and novel sequences for male sterility

An expression cassette comprising an Atp8-1 polynucleotide associated with a male sterile phenotype, wherein the polynucleotide is selected from:

• (a) a nucleotide sequence comprising SEQ ID NO:
• 66;
• (b) a nucleotide sequence encoding a polypeptide comprising SEQ ID NO:
• 70;
• (c) a nucleotide sequence comprising at least 85% sequence identity to SEQ ID NO:
• 66 and one or more of the following:
• a C at position 56, a C at position 77, an A at position 87, or a C at position 238;
• (d) a nucleotide sequence encoding a polypeptide comprising at least 85% identity to SEQ ID NO:
• 70 and one or more of the following:
• a P residue at position 19, a P residue at position 26, a K residue at position 29, or a P residue at position 80.

An expression cassette comprising an NAD9 polynucleotide associated with a male sterile phenotype, wherein the polynucleotide is selected from:

• (a) a nucleotide sequence comprising SEQ ID NO:
• 68;
• (b) a nucleotide sequence encoding a polypeptide comprising SEQ ID NO:
• 72;
• (c) a nucleotide sequence comprising at least 85% sequence identity to SEQ ID NO:
• 68 and one or more of the following:
• a C at position 118, an A at position 135, or a G at position 286;
• (d) a nucleotide sequence encoding a polypeptide comprising at least 85% identity to SEQ ID NO:
• 72 and one or more of the following:
• an H residue at position 40, an A residue at position 45, or an A residue at position 96.

A method for producing a male sterile plant, the method comprising:

• introducing one or more mutations into an endogenous Atp8-1 or NAD9 nucleic acid sequence, wherein the one or more mutations are selected from:
• a C at position 56, a C at position 77, an A at position 87, or a C at position 238 when compared to wild type reference SEQ ID NO:
• 65;
• or a C at position 118, an A at position 135, or a G at position 286 when compared to wild type reference SEQ ID NO:
• 67.

A method of identifying a male sterile plant, the method comprising:

• assaying the plant for an Atp8-1 or NAD9 nucleic acid sequence associated with a male sterile phenotype comprising one or more of the following:
• a C at position 56, a C at position 77, an A at position 87, or a C at position 238 when compared to wild type reference SEQ ID NO:
• 65;
• or a C at position 118, an A at position 135, or a G at position 286 when compared to wild type reference SEQ ID NO:
• 67.