US 2023/0413749 A1 · Method for Inducing Lateral Branch Development of Eucheuma Denticulatum

Abstract

The present disclosure provides a method for inducing a lateral branch development of Eucheuma denticulatum, and relates to the technical field of marine organisms. In the present invention, the E. denticulatum is subjected to aeration culture at a light-photon flux of 200 μmol·m−2·s−1 to 300 μmol·m−2·s−1. During induction with a high light intensity, new lateral branches continue to grow; after the high-light treatment is completed, the E. denticulatum is placed in its original breeding environment. The dedifferentiation of branch cells into meristems is induced by the high light intensity in E. denticulatum, and then the meristems are developed into lateral branches. An increase of the lateral branches can increase a yield of E. denticulatum, and the high-light treatment can significantly increase a dry matter content of the E. denticulatum to raise an income.

Claims4

Dependent claims

A method for inducing a lateral branch development of Eucheuma denticulatum, comprising the following steps:

conducting aeration culture on E. denticulatum under light illumination of 300 μmol·m−2·s−1;
wherein
the aeration culture is conducted in a Provasoli's enrichment solution (PES) medium;
the light illumination comprises 12 h continuous light and 12 h continuous darkness every day;
the PES medium comprises the following components:
2.35 g/L of NaNO3, 0.35 g/L of C3H7Na2O6P·5H2O, 162.5 mL/L of an ES Fe solution, 162.5 mL/L of a P-II metal solution, and 3.25 g/L of a HEPES buffer;
the ES Fe solution comprises the following components by concentration:
0.7 g/L of Fe(NH4)2(SO4)2·6H2O and 0.6 g/L of Na2EDTA·2H2O;
and
the P-II metal solution comprises the following components by concentration:
1 g/L of Na2EDTA·2H2O, 1.14 g/L of H3BO3, 0.049 g/L of FeCl3·6H2O, 0.164 g/L of MnSO4·H2O, 0.022 g/L of ZnSO4·7H2O, and 0.0048 g/L of CoCl2·6H2O.

8 more elements

Dependencies are inferred from the claim text.

Method for Inducing Lateral Branch Development of Eucheuma Denticulatum

Life of this patent

Method for Inducing Lateral Branch Development of Eucheuma Denticulatum

Life of this patent

Abstract

Claims4