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US 2023/0413734 A1Published application
Method of producing interspecific hybrids between cross-incompatible plants
AssigneeUniversity of Louisiana Lafayette
InventorsYi-Hong WANG, Chudamani Sharma Prakash, Jieqin Li
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Abstract
This inventive method comprises regeneration of a hybrid plant via grafting between cross-incompatible species. By grafting and regenerating hybrid from the graft junction in selection media, the graft junction will regenerate hybrid plants with selection for the presence of both species.
Claims12
A method for grafting plants comprising:
- a. Identifying a selection marker on a first plant and a second plant, wherein said first plant and said second plant are of difference species, and wherein said first plant and said second plant each comprise:
- embryonic tissues from seeds of said each of said first plant and said second plant, a rootstock, and a scion;
- b. Soaking said seeds of said first plant and said seeds of said second plant under sterile conditions for 2-48 hours to initiate germination;
- c. Removing plumules from said seeds of said first plant and removing plumules from said seeds of said second plant;
- d. Attaching the scion of said first plant to the rootstock of said second plant to create a first graft and the scion of said second plant to the rootstock of said first plant to create a second graft;
- e. Resting said first graft and said second graft under moist and sterile conditions for 5-10 days so that said first graft becomes a live first graft and said second graft becomes a live second graft;
- f. Transferring said live first graft and said live second graft to a culture medium and subjecting said transferred live grafts to a diurnal cycle of 16 hours of light and 8 hours of dark at 25° C. to create a growing first graft and a growing second graft;
- and
- g. Transferring said growing first graft and said growing second graft to a regeneration medium comprising selection agents and allowing regeneration for 1-4 months to create a regenerated first graft and a regenerated second graft.
6 more elements
A method for grafting plants comprising:
- a. Identifying a selection marker on a first plant and a second plant, wherein said first plant and said second plant are of difference species, and wherein said first plant and said second plant each comprise:
- embryonic tissues from seeds of said each of said first plant and said second plant, a rootstock, and a scion;
- b. Soaking said seeds of said first plant and said seeds of said second plant under sterile conditions for 2-48 hours to initiate germination;
- c. Removing plumules from said seeds of said first plant and removing plumules from said seeds of said second plant;
- d. Attaching the scion of said first plant to the rootstock of said second plant to create a graft;
- e. Resting said graft under moist and sterile conditions so that said graft becomes a live graft;
- f. Transferring said live graft to a culture medium and subjecting said transferred live grafts to a diurnal cycle to create a growing graft;
- and
- g. Transferring said growing graft to a regeneration medium comprising selection agents and allowing regeneration to create a regenerated graft.
6 more elements
A method of grafting between cross-incompatible plants to regenerate hybrid plants that combine both parental genomes comprising:
- a. Soaking seeds of a first plant and the seeds of a said second plant under sterile conditions for 2-48 hours to initiate germination;
- b. Removing plumules from said seeds of said first plant and removing plumules from said seeds of said second plant;
- c. Attaching the scion of said first plant to the rootstock of said second plant to create a graft;
- d. Resting said graft under moist and sterile conditions so that said graft becomes a live graft;
- e. Transferring said live graft to a culture medium and subjecting said transferred live grafts to a diurnal cycle to create a growing graft;
- f. Transferring said growing graft to a regeneration medium comprising selection agents and allowing regeneration to create a regenerated graft;
- and
- g. Transferring said regenerated graft to rooting media to create a hybrid plant.
5 more elements
Dependencies are inferred from the claim text.