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US 2023/0408533 A1Published application
Rapid on-site detection method for gamma-hydroxybutyric acid
AssigneeNortheastern University
InventorsYunrong CHAI, Yuxuan Qin, Nicole Taylor Cavanaugh
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Abstract
Disclosed are compositions and methods for detecting the presence or level of gamma-hydroxybutyric acid (GHB), or for diagnosing GHB toxicity or overdose.
Claims25
A kit for detecting the presence or the level or concentration of GHB in a biological sample, comprising:
- (1) a recombinant GHB dehydrogenase (GHBDH) enzyme;
- (2) a recombinant NADH-specific diaphorase;
- and
- (3) a dye.
1 more element
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A method of detecting the presence or the level or concentration of GHB in a biological sample, comprising:
- (a) contacting the biological sample with:
- (1) a recombinant GHBDH enzyme;
- (2) a recombinant NADH-specific diaphorase;
- and
- (3) a dye;
- (b) comparing the color of the reaction mix to a standard colorimetric paper strip to determine the concentration of GHB in the biological sample.
3 more elements
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A recombinant E. coli strain, comprising a nucleic acid comprising a nucleotide sequence encoding GHBDH, and/or a nucleotide sequence encoding NADH-specific diaphorase.
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Dependencies are inferred from the claim text.