Sandwich assays in droplets
AssigneeBio-Rad Laboratories, Inc.
InventorsMichael L. Samuels, Darren Roy Link
Life of this patent
Estimating the term…
Abstract
The invention generally relates to performing sandwich assays in droplets. In certain embodiments, the invention provides methods for detecting a target analyte that involve forming a compartmentalized portion of fluid including a portion of a sample suspected of containing a target analyte and a sample identifier, a first binding agent having a target identifier, and a second binding agent specific to the target analyte under conditions that produce a complex of the first and second binding agents with the target analyte, separating the complexes, and detecting the complexes, thereby detecting the target analyte.
Claims21
1-20. (canceled)
A method comprising:
- encapsulating cells and binding agents comprising oligonucleotide-linked binders into a plurality of partitions to create at least one partition containing a single cell, wherein each binding agent is provided with an oligonucleotide sequence that includes an analyte identifier specific for a target analyte and a unique identifier unique to the binding agent;
- lysing the cell within the partition to release the target analyte;
- binding one of the binding agents to the target analyte;
- and
- sequencing the oligonucleotide sequence to detect the target analyte.
A method comprising:
- encapsulating cells into a plurality of partitions to create at least one partition containing a single cell, wherein the partition includes one or more binding agents, wherein the binding agents include antibodies bound to an analyte identifier for a target analyte and an identifier specific for the partition;
- lysing the cell within the partition to release the target analyte;
- binding the one or more binding agents to the target analyte;
- and
- detecting the target analyte identifier, thereby detecting the target analyte.
Dependencies are inferred from the claim text.