Flow based assays for therapeutics

A method of analyzing a response to therapeutic antibodies, the method comprising:

• exposing a first aliquot of whole blood sample to a first therapeutic antibody and a second aliquot of whole blood sample to a second therapeutic antibody;
• combining exposed blood cells from the whole blood sample with a panel of labeled reporters, the panel of labeled reporters comprising at least two labeled reporters selected from the group consisting of a labeled reporter directed against a target cell depletion surface marker, a labeled reporter directed against a target cell activation surface marker expressed on white blood cell populations, and a labeled reporter directed against cytokine production by white blood cells to thereby simultaneously produce assay signals generated by each for the at least two labeled reporters;
• subjecting the whole blood cell sample mixtures to flow cytometry and measuring signals generated by each of the plurality of labeled cells of each aliquot in a flow cytometer;
• and
• combining the measured signals into an assay output for flow cytometric multiparameter analysis to determine a cellular response to the first therapeutic antibody and to the second therapeutic antibody and provide simultaneous differentiation between signals associated with one or more of target cell depletion, activation of white cell subpopulations, and cytokine production by white cells.