Device and process for analysing gas effects in samples
AssigneesMedizinische Universität Innsbruck, Eurac Research
InventorsSimon Woyke, Thomas Haller
Life of this patent
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Abstract
The invention provides a device and a process for analysing the effect of a concentration of a gas on a sample, especially measurement of the gas concentration within a sample, and/or measurement of the effect of the absorption and/or desorption of a gas by the sample, using spectrophotometric detection of a gas-sensitive and optically detectable analyte of the sample while measuring the concentration of the gas in a gas composition that is continuously delivered to each sample well in order to contact the samples with a gas having a predetermined and/or measured concentration, respectively a pre-determined and/or measured partial pressure.
Claims20
A device for analyzing the effect of a gas in a sample, the device comprising:
- a housing enclosing at least one sample well, a gas conduit connected to the at least one sample well configured to permit gas to enter and to exit the at least one sample well, a gas source connected to an inlet of the gas conduit, a concentration device that is configured to determine the concentration of at least one component of the gas, and a spectrophotometer arranged to optically measure the at least one sample well.
A process for analyzing the effect of a gas comprising depositing the at least one sample into one of an array of at least two sample wells contained in a housing, continuously delivering a gas composition containing a gas to the array of sample wells through a gas conduit in connection with each sample well, and spectrophotometrically concurrently measuring each sample well containing an aliquot of samples while in contact with gas.
A process for producing a device for analyzing the effect of a gas in a sample, comprising providing a microtiter plate reader as a spectrophotometer, arranging a housing comprising an array of sample wells connected in series by a gas conduit with its inlet connected to a gas source, the housing comprising an oxygen-sensitive dye arranged in a well of the housing as a first gas sensor, which well is arranged between the inlet and sample wells, and comprising an oxygen-sensitive dye arranged in a well of the housing as a second gas sensor, which well is arranged between sample wells and an outlet of the gas conduit, in a measuring chamber of the microtiter plate reader, and arranging a closure element for closing an opening of a measuring chamber, which closure element accommodates at least a gas feed line connected to the inlet of the gas conduit.
Dependencies are inferred from the claim text.