Products and processes for multiplex nucleic acid identification

A method for detecting the presence, absence or amount of a plurality of genetic variants in a composition, comprising:

• (a) preparing a plurality of amplicons derived from a plurality of target nucleic acid species, or portions thereof, wherein each target nucleic acid species comprises a first variant and a second variant;
• (b) hybridizing the amplicons to oligonucleotide species, wherein each oligonucleotide species hybridizes to an amplicon derived from a target nucleic acid species, thereby generating hybridized oligonucleotide species;
• and
• (c) contacting the hybridized oligonucleotide species with an extension composition comprising one or more terminating nucleotides under extension conditions;
• wherein:
• (i) at least one of the one or more terminating nucleotide comprises a capture agent, and
• (ii) the hybridized oligonucleotide species that hybridize to the first variant are extended by a terminating nucleotide and the hybridized oligonucleotide species that hybridize to the second variant are not extended by a terminating nucleotide, thereby generating extended oligonucleotide species;
• (d) capturing the extended oligonucleotide species to a solid phase that captures the capture agent;
• (e) releasing the extended oligonucleotide species bound to the solid phase in (d) from the solid phase;
• and
• (f) detecting the mass of each extended oligonucleotide species released from the solid phase in (e) by mass spectrometry;
• whereby the presence, absence or amount of the genetic variants is detected.