Long-acting recombinant interleukin-18 binding protein and production method and application thereof

A method for fermenting and producing a recombinant interleukin-18 binding protein (IL-18BP), comprising:

• inducing fermentation to culture a host bacterium to obtain the recombinant interleukin-18 binding protein;
• wherein expression conditions for the inducing with isopropyl-β-D-thiogalactoside (IPTG) are as follows:
• induction at 0.5 millimoles per liter (mmol/L) IPTG at 20 Celsius degree (° C.) for 4-31 hours;
• wherein the recombinant IL-18BP is prepared as follows:
• connecting a 5′ end of a coding gene of the recombinant IL-18BP with a gene sequence of a molecular chaperone Sumo to construct a fusion gene, inserting the fusion gene into a cell expression vector, and guiding the cell expression vector into a prokaryotic cell to express the recombinant IL-18BP;
• wherein the recombinant IL-18BP comprises a sequence encoding human IL-18BP isoform a (hIL-18BPa) and a sequence encoding human immunoglobulin class G-crystallizable fragment (IgG-Fc);
• an amino acid sequence of the recombinant IL-18BP is shown in SEQ ID NO:
• 1, a nucleotide sequence of the coding gene of the recombinant IL-18BP is shown in SEQ ID NO:
• 3;
• and an amino acid sequence of the molecular chaperone Sumo is shown in SEQ ID NO:
• 2.