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US 2023/0407256 A1Published application
Generation of therapeutic cells using extracellular components of target organs
AssigneeWisconsin Alumni Research Foundation
InventorsPeiman Hematti, Eric G. Schmuck, John A. Kink, Amish N. Raval
Life of this patent
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Abstract
The invention relates to an ex vivo generated population of tissue-specific anti-inflammatory macrophages and methods of making and using such macrophages.
Claims14
1-20. (canceled)
A method for generating an anti-inflammatory macrophage, the method comprising the step of:
- co-culturing a population of isolated CD14+ cells with isolated cardiac fibroblast-derived extracellular matrix (CF-ECM) in vitro until the population of isolated CD14+ cells acquires an anti-inflammatory macrophage phenotype, wherein the population of anti-inflammatory macrophages comprises cardiac fibroblast-derived extracellular matrix educated macrophages (CF-ECM-EM) that express CD163 high, CD206 high, PD-L1 high, CD68 low, HLA-DR low, and CD86 low as compared to uneducated macrophages.
Dependencies are inferred from the claim text.