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AssigneeBgi Shenzhen Group Holdings Co., Limited
InventorsMiguel A. Esteban, Md. Abdul Mazid, Wenjuan Li, Zhiwei Luo, Carl Ward, Yunpan Li, Yiwei Lai
Estimating the term…
Provided are media and methods for establishing and maintaining mammalian early embryo-like cells. The culture media can be used to culture mammalian pluripotent stem cells (PSCs), which is chemically defined and comprises basal media for culturing stem cells supplemented with a S-adenosylhomocysteine hydrolase (SAH)/Polycomb repressive complexes (PRC)/EZH2 inhibitor, a histone deacetylase (HDAC) inhibitor and a WNT/β-catenin signaling/tankyrase inhibitor. With the culture media, primate (human and non-human) PSCs can be converted to preimplantation ICM-like cells (ICLCs) or 8-cell embryo-like cells (8CLCs).
A chemically defined culture medium for culturing PSCs comprising a basal medium for culturing stem cells supplemented with a SAH/PRC/EZH2 inhibitor, a HDAC inhibitor, and a WNT/β-catenin signaling inhibitor.
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A method for converting primate PSCs to one or more of ICLCs and 8CLCs or for converting ICLCs to 8CLCs, comprising culturing the primate PSCs or JCLCs in the presence of a SAH/PRC/EZH2 inhibitor, a HDAC inhibitor and a WNT/β-catenin signaling inhibitor;
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A kit comprising a SAH/PRC/EZH2 inhibitor, a HDAC inhibitor and a WNT/β-catenin signaling inhibitor, and optionally
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A composition comprising a SAH/PRC/EZH2 inhibitor, a HDAC inhibitor, and a WNT/β-catenin signaling inhibitor and optionally
A method for reprogramming somatic cells to ICLCs, promoting conversion of primate PSCs to ICLCs, or for promoting conversion of primate one or more of PSCs and ICLCs to 8CLCs, comprising:
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Dependencies are inferred from the claim text.