Tetrahydrocannabinolic acid (thca) synthase variants, and manufacture and use thereof
OwnerJasleen BAINES, Oleg TYURIN +2inventors
InventorsJasleen BAINES, Oleg TYURIN, Malcolm J. KAVARANA, Mingyang SUN
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Abstract
Provided herein is a recombinant vector that comprises a promoter, a secretion tag gene of interest (GOI), and optionally a purification and/or a detection tag. The GOI comprises a polynucleotide sequence as set forth in any one of SEQ ID NOs: 2-57 or a polynucleotide sequence with at least 90%, 95%, 98%, 99% or 99.9% identity with any one of SEQ ID NOs: 2-57. Also, disclosed are the sequences of polypeptides that result from expression of a GOI by a host expression system, and a method of producing cannabinoid compounds.
Claims20
A recombinant vector comprising a promoter, secretion tag, gene of interest (GOI), and optionally a purification and/or a detection tag, wherein the gene of interest comprises a polynucleotide sequence encoding a polypeptide with at least 90%, 95%, 98%, or 99.9% sequence identity to the sequence as set forth in any one of SEQ ID NOs:
- 84-139.
A polypeptide comprising the amino acid sequence of any one of SEQ ID NOs:
- 84-139 or an amino acid sequence comprising at least 90%, 95%, 98%, or 99.9% sequence identity to any one of SEQ ID NOs:
- 84-139.
A nucleic acid comprising a polynucleotide sequence encoding a polypeptide comprising the amino acid sequence of any one of SEQ ID NOs:
- 84-139 or an amino acid sequence comprising at least 90%, 95%, 98%, or 99.9% sequence identity to any one of SEQ ID NOs:
- 84-139. The nucleic acid of claim 19, wherein the nucleic acid comprises the polynucleotide sequence of any one of SEQ ID NOs:
- 2-57 or a polynucleotide sequence comprising at least 90%, 95%, 98%, or 99.9% sequence identity to any one of SEQ ID NOs:
- 2-57.
The nucleic acid of claim 20, wherein the nucleic acid comprises the polynucleotide sequence of any one of SEQ ID NOs:
- 9 and 28-34 or a polynucleotide sequence comprising at least 90%, 95%, 98%, or 99.9% sequence identity to any one of SEQ ID NOs:
- 9 and 28-34.
Dependencies are inferred from the claim text.