Factory-on-a-chip for production of biologically derived medicines/biopharmaceuticals/biologics/ biotherapeutics
AssigneeUniversity of Maryland, Baltimore County
InventorsGOVIND RAO, YORDAN KOSTOV, ABHAY ANDAR, MUSTAFA AL-ADHAMI, SEVDA DELDARI, DOUGLAS D. FREY
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Abstract
The present invention provides for a fully integrated microfluidic system capable of producing single-dose amounts of biotherapeutics at the point-of-care wherein protein production, purification and product harvest are all integrated as a single microfluidic device which is portable and capable of continuous-flow production of biotherapeutics at the microscale using a cell-free reaction system.
Claims20
A method of administering a purified therapeutic protein to a subject in need of said therapeutic protein, said method comprising:
- synthesizing and purifying the therapeutic protein in a factory-on-a-chip microfluidic device;
- and
- administering the purified therapeutic protein to the subject in a sufficient amount within 24 hours of purification.
A factory-on-a-chip microfluidic device comprising at least three separate units that can be stacked together to form a single unit, wherein the separate units comprise:
- (i) a microfluidic bioreactor unit equipped with a continuous collection channel for synthesizing a crude protein in a reaction within the microfluidic bioreactor unit;
- (ii) a microfluidic mixer/de-bubbler unit communicatively connected to the microfluidic bioreactor unit to dilute the crude protein and remove any air bubbles during mixing;
- and
- (iii) a microfluidic purification unit comprising at least one purification column, communicatively connected to and downstream of the microfluidic mixer/de-bubbler unit, for capturing the crude protein and providing a purified protein.
Dependencies are inferred from the claim text.