Configurable substrate of a fluidic device
AssigneeXbiologix, Inc.
InventorsRyan A. MEHL, Eduardo CEBALLOS, Kevin F. JONES
Life of this patent
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Abstract
A method of forming a configurable substrate includes depositing a volume of trans-cyclooctene on a configurable substrate of a fluidic device, and forming a bioorthogonal tethered protein on the configurable substrate. The bioorthogonal tethered protein may be formed by attaching a tetrazine-modified protein or tetrazine-modified functional protein fragment to the trans-cyclooctene, wherein the tetrazine-modified protein or tetrazine-modified functional protein fragment is configured to bind to a target analyte.
Claims21
1-20. (canceled)
A method, comprising:
- forming a bioorthogonal tethered protein on a configurable substrate by attaching a tetrazine-modified protein or a tetrazine-modified functional protein fragment to trans-cyclooctene, wherein the bioorthogonal tethered protein includes a ligand configured to bind to a target analyte and wherein a concentration, a length, and an orientation of the bioorthogonal tethered protein are configurable on the configurable substrate.
A configurable substrate of a fluidic device, comprising:
- an assay region of the configurable substrate including a bioorthogonal tethered protein, wherein the bioorthogonal tethered protein includes:
- a volume of trans-cyclooctene tethered to a surface of the configurable substrate;
- and
- a tetrazine-modified protein or tetrazine-modified functional protein fragment tethered to the trans-cyclooctene, wherein the tetrazine-modified protein or tetrazine-modified functional protein fragment includes a ligand configured to bind to a target analyte, wherein at least one of a concentration, a length, and an orientation of the bioorthogonal tethered protein are configured on the configurable substrate.
A method, comprising:
- dispensing a volume of trans-cyclooctene on an assay region of a configurable substrate of a fluidic device;
- and
- forming a bioorthogonal tethered protein on the configurable substrate by attaching a tetrazine-modified protein or tetrazine-modified functional protein fragment to the trans-cyclooctene, wherein the tetrazine-modified protein or tetrazine-modified functional protein fragment is configured to bind to a target analyte.
Dependencies are inferred from the claim text.