Alginate Based Particles as a Temporary Embolic Agent
AssigneeCrannmed Limited
InventorsSankalp Agarwal, Masum Pandey, Andrew Lewis
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Abstract
The present disclosure provides compositions including alginate microspheres capable of self-degradation upon rehydration, the alginate microspheres comprising alginate, alginate lyase, and divalent metal ions. The present disclosure also provides methods of making compositions including alginate microspheres capable of self-degradation upon rehydration, comprising alginate, alginate lyase, and divalent metal ions. The present disclosure also provides methods of inducing an embolism in a subject in thereof, and syringes containing the compositions of the present disclosure for use in the methods thereof.
Claims21
An alginate microsphere capable of self-degradation upon rehydration, comprising:
- an alginate lyase enzyme pre-treated by varying temperature, by varying pH, and/or with a metal-ion enzyme inhibitor;
- alginate molecules having one or both of (i) a predetermined molecular weight, and (ii) a predetermined ratio of β-D-Mannuronic acid (M) blocks to α-L-Guluronic acid (G) blocks;
- and
- a divalent metal-ion crosslinking the alginate molecules,
- wherein the alginate microsphere is substantially free of water and/or sterilized.
A method of preparing an alginate microsphere capable of self-degradation upon rehydration, the method comprising:
- forming droplets from a precursor solution, the precursor solution comprising:
- an alginate lyase enzyme pre-treated by varying temperature, by varying pH, and/or with a metal-ion enzyme inhibitor;
- and
- alginate molecules having one or both of (a) a predetermined molecular weight, and (b) a predetermined ratio of β-D-Mannuronic acid (M) blocks to α-L-Guluronic acid (G) blocks;
- contacting the droplets with a gelling bath comprising a divalent metal-ion, thereby crosslinking the alginate molecules to form an alginate microsphere;
- and
- dehydrating, and optionally sterilizing, the alginate microsphere thereby substantially removing water from the microsphere.
Dependencies are inferred from the claim text.