Ultrashort tetrameric peptide nanogels support tissue graft formation, wound healing and 3d bioprinting

A method of wound treatment comprising:

• applying a material to a subject,
• wherein the material comprises an ultrashort self-assembling amphiphilic peptide wherein said aliphatic amino acids and said aromatic amino acids and said polar amino acids are either D amino acids or L amino acids, comprising:
• at least one peptide selected from a group of peptides having a general formula selected from AnBmX, BmAnX, XAnBm, and XBmAn,
• wherein the total number of amino acids of the ultrashort self-assembling amphiphilic peptide wherein said aliphatic amino acids and said aromatic amino acids and said polar amino acids are either D amino acids or L amino acids, does not exceed 7 amino acids;
• wherein A is an aliphatic amino acids, selected from the group consisting of:
• isoleucine, leucine, norleucine, cyclohexylalanine, valine, alanine, glycin, homoallylglycine and homopropargylglycine or any combination thereof, with n being an integer being selected from 0-5;
• wherein B is comprised of at least one aromatic amino acid selected from the group consisting of:
• tyrosine, tryptophan, phenylalanine, L-DOPA, or comprised of a peptidomimetic amino acid that is the aliphatic counterpart of the aromatic amino acid, which is the counterpart of amino acid phenylalanine with m being an integer being selected from 0-3;
• and
• wherein X is comprised of a polar amino acid, selected from the group consisting of:
• aspartic acid, glutamic acid, lysine, arginine, histidine, cysteine, serine, threonine, asparagine, and glutamine, 5-N-ethyl glutamine (theanine), citrulline, thio citrulline, homocysteine, methionine, ethionine, selenomethionine, telluromethionine, allo threonine, homoserine, homoarginine, ornithine, N(6) carboxymethyllysine, or any combination thereof, where the polar group is selected from a hydroxyl, an ether, a carboxyl, an imido, an amido, an amino, an ester, a guanidino, a thio, a thioether, a seleno, and a telluro group.

A method of preparing a hydrogel or organogel comprising dissolving a peptide in an aqueous solution or an organic solution, respectively, wherein the peptide comprises:

• at least one peptide selected from a group of peptides having a formula selected from AnBmX, BmAnX, XAnBm, and XBmAn,
• wherein the total number of amino acids of the ultrashort self-assembling does amphiphilic peptide wherein said aliphatic amino acids and said aromatic amino acids and said polar amino acids are either D amino acids or L amino acids, not exceed 7 amino acids;
• wherein A is an aliphatic amino acids, selected from the group consisting of:
• isoleucine, leucine, norleucine, cyclohexylalanine, valine, alanine, glycin, homoallylglycine and homopropargylglycine or any combination thereof, with n being an integer being selected from 0-5;
• wherein B is comprised of at least one aromatic amino acid selected from the group consisting of:
• tyrosine, tryptophan, phenylalanine, L-DOPA, or comprised of a peptidomimetic amino acid that is the aliphatic counterpart of the aromatic amino acid, which is the counterpart of amino acid phenylalanine with m being an integer being selected from 0-3;
• and
• wherein X is comprised of a polar amino acid, selected from the group consisting of:
• aspartic acid, glutamic acid, lysine, arginine, histidine, cysteine, serine, threonine, asparagine, and glutamine, 5-N-ethyl glutamine (theanine), citrulline, thio citrulline, homocysteine, methionine, ethionine, selenomethionine, telluromethionine, allo threonine, homoserine, homoarginine, ornithine, N(6) carboxymethyllysine, or any combination thereof, where the polar group is selected from a hydroxyl, an ether, a carboxyl, an imido, an amido, an amino, an ester, a guanidino, a thio, a thioether, a seleno, and a telluro group.