Method for preparing nanoparticles
OwnerOlivier TILLEMENT, François LUX +3inventors
InventorsOlivier TILLEMENT, François LUX, Fabien ROSSETTI, Paul ROCCHI, Tristan DOUSSINEAU
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Abstract
The present disclosure relates to nanoparticles and the uses thereof in medicine, in particular for the treatment of tumours.
Claims10
A process for preparing a colloidal solution of nanoparticles, each nanoparticle comprising chelating groups grafted onto a polymer matrix, one portion only of the chelating groups being complexed with a metal cation, the other portion being uncomplexed, said process comprising
- (1) the synthesis or the provision of a colloidal solution of precursor nanoparticles, said precursor nanoparticles having the following formula [Ch-M1]n-PS wherein:
- PS is an organic or inorganic polymer matrix, for example a polysiloxane matrix,
- [Ch-M1] is a chelating group complexed with a metal cation M1 with a high atomic number Z greater than 40, and preferably greater than 50,
- Ch is covalently grafted to the surface of the polymer matrix, for example, a polysiloxane matrix,
- n is between 5 and 100, and,
- the average hydrodynamic diameter of the nanoparticles is between 1 and 50 nm, preferably between 2 and 20 nm, and more preferentially between 2 and 8 nm,
- (2) a step of treating the colloidal solution in an acid medium, for example by adding a hydrochloric acid solution, in order to obtain a pH preferably below 2.0, preferably below 1.0, for a time sufficient to obtain a partial release of the metal cations M1,
- (3) where appropriate, a step of diluting the colloidal solution, for example with water,
- (4) a purification step to separate the nanoparticles obtained in step (2) from the metal cations M1 released,
- (5) where appropriate, a step of concentrating the solution of the nanoparticles obtained in step (4),
- (6) where appropriate, repeating steps (3), (4) and (5),
- (7) where appropriate, freezing and/or freeze-drying the solution of nanoparticles obtained in one of steps (4), (5) or (6).
A process for preparing a colloidal solution of nanoparticles, each nanoparticle comprising chelating groups grafted onto a polymer matrix, a first fraction f1 of the chelating groups being complexed with a metal cation M1, a second fraction f2 being being-complexed with a cation M2, and a third fraction f3 being uncomplexed, said process comprising
- (1) the synthesis or the provision of a colloidal solution of precursor nanoparticles, said precursor nanoparticles having the following formula [Ch-M1]n-PS wherein:
- PS is an organic or inorganic polymer matrix,
- Ch is a chelating group complexed with a metal cation M1 with a high atomic number Z greater than 40, and preferably greater than 50,
- Ch is grafted onto the polymer matrix,
- n is between 5 and 100, and,
- the average hydrodynamic diameter of the nanoparticle is between 1 and 50 nm, preferably between 2 and 20 nm, and more preferentially between 2 and 8 nm
- (2) a step of treating the colloidal solution in an acid medium, for example by adding a hydrochloric acid solution, in order to obtain a pH below 2.0, preferably below 1.0, for a time sufficient to obtain a partial release of the metal cations M1,
- (3) where appropriate, a step of diluting the solution, for example with water,
- (4) a purification step to separate the nanoparticles obtained in step (2) from the free metal cations M1,
- (5) where appropriate, a step of concentrating the solution of the nanoparticles obtained in step (4),
- (6) where appropriate, repeating steps (3), (4) and (5),
- (7) optionally, a step of partial recomplexation of the nanoparticles obtained in step (2), (3), (4), (5) or (6) with a determined amount of metal cation M1 in order to obtain a determined amount of chelating group Ch complexed with the metal cation M1,
- (8) bringing the solution of nanoparticles obtained in step (4), (5), (6) or (7) into contact with a sufficient amount of cation M2, preferably with a high atomic number Z greater than 40, and preferably greater than 50, for example a metal cation different from the metal cations M1 or a radioisotope, to complex at least some of the chelating groups Ch1 freed in step (2) and,
- (9) where appropriate, freezing and/or freeze-drying the solution of nanoparticles obtained in step (8).
Dependencies are inferred from the claim text.