Back to results
US 2023/0405142 A1Published application
Protein-templated self-assembly of a covalent polymer network for intracellular trafficking and traceless release
AssigneeUniversity of Massachusetts
InventorsSankaran Thayumanavan, Jiaming Zhuang
Life of this patent
Estimating the term…
Abstract
The invention provides polymers and polymer-based nano-structures, in particular, polymers and polymer network to which biomolecules (e.g., proteins, antibodies, peptide aptamers) can be covalently conjugated and stably encapsulated therein and be controllably delivered and released upon degradation of the nano-structures in response to specific microenvironment, and compositions and methods of preparation and use thereof.
Claims26
1-31. (canceled)
A method for controlled delivery of a protein to a target biological site inside a cell, comprising:
- providing a nano-assembly comprising a polymer-protein conjugate wherein the protein is encapsulated in and covalently conjugated to a crosslinked polymer network;
- delivering the nano-assembly intracellularly to the target biological site;
- and
- causing at least partial dissociation of the nano-assembly and release of the protein therefrom resulting in intracellular release of the protein at the target biological site,
- wherein the nano-assembly is formed by
- reacting one or more side chain functionalities of the polymer with one or more surface-exposed functional groups of one or more lysine residues of the protein;
- and
- encapsulating the protein in the polymer-protein conjugate by forming a crosslinked polymer network around the conjugated protein.
5 more elements
(canceled)
(canceled)
(canceled)
(canceled)
56-81. (canceled)
Dependencies are inferred from the claim text.