High speed scanning system with acceleration tracking
AssigneeApton Biosystems, Inc.
InventorsWindsor OWENS, Bryan P. STAKER, Robert HARTLAGE, Edvinas ZIZMINSKAS, David STERN, Paul HEILMAN
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Abstract
Disclosed herein is a high throughput optical scanning device and methods of use. The optical scanning device and methods of use provided herein can allow high throughput scanning of a continuously moving object with a high resolution despite fluctuations in stage velocity. This can aid in high throughput scanning of a substrate, such as a biological chip comprising fluorophores. Also provided herein are improved optical relay systems and scanning optics.
Claims21
-26. (canceled)
An optical scanning system for imaging a moving substrate, comprising:
- (a) a stage, said stage capable of moving along an axis, said stage configured to hold a substrate comprising a plurality of fields;
- (b) an objective lens;
- (c) a camera capable of capturing an image of one of said plurality of fields through said objective lens, said image acquired via an optical path defined from one of said plurality of fields through said objective lens to said camera during acquisition of said image;
- (d) a motion tracking mirror mounted along said light path;
- (e) an electric motor operably coupled to said motion tracking mirror to actuate angular motion of said tracking mirror along said axis of stage movement in said optical path;
- and
- (f) a controller module operably coupled to said electric motor to send a driving signal to said electric motor, wherein said controller module is capable of generating said driving signal as a function of a velocity fluctuation of said stage or substrate movement along said axis.
A method of imaging a plurality of fields on a moving substrate, comprising:
- (a) providing an optical scanning system comprising:
- (i) a stage configured to hold a substrate comprising a plurality of fields;
- (ii) an objective lens;
- (iii) a camera configured to capture an image of one of said plurality of fields through said objective lens, said image acquired via an optical path defined from one of said plurality of fields through said objective lens to said camera during acquisition of said image;
- (iv) a motion tracking mirror mounted along said light path;
- and
- (v) an electric motor operably coupled to said motion tracking mirror to actuate angular motion of said motion tracking mirror along said axis of stage movement in said optical path;
- and
- (vi) a controller module operably coupled to said electric motor to send a driving signal to said electric motor, wherein said controller module is capable of generating said driving signal as a function of a velocity fluctuation of said stage or substrate movement along said axis;
- (b) moving said substrate with said stage along an axis;
- and
- (c) actuating said electric motor operably coupled to said motion tracking mirror with said driving signal;
- and
- (d) capturing said image of said field of said plurality of fields with said camera.
Dependencies are inferred from the claim text.