US 2023/0400461 A1 · METHODS, ANTIBODIES OR ANTIGEN BINDING FRAGMENTS AND OTHER TOOLS FOR DETERMINING TARGET MOLECULES

Abstract

The invention relates to a method for determining a target molecule in a sample. Also, the invention relates to a Förster resonance energy transfer (FRET, fluorescence resonance energy transfer) pair, recombinant antibodies, or antigen binding fragments thereof that bind Staphylococcus aureus Enterotoxin A and a test kit comprising the FRET pair, antibody or antigen binding fragment. Furthermore, the present invention relates to an isolated nucleic acid molecule comprising a nucleotide sequence that encodes the recombinant antibody or antigen binding fragment, and an expression vector comprising said nucleic acid molecule. Still, the invention relates to use of the FRET pair or the recombinant antibody or an antigen binding fragment thereof for determining a target molecule such as Staphylococcus aureus Enterotoxin A in a sample. And still, the invention relates to a method of producing the antibody or antigen binding fragment.

Claims37

Dependent claims

A method for determining a target molecule in a sample, wherein the method comprises:

allowing a first recombinant antibody or antigen binding fragment thereof, which is capable of binding a target molecule, and a second recombinant antibody or antigen binding fragment thereof, which is capable of binding said target molecule at one or more different binding sites compared to the first recombinant antibody or antigen binding fragment thereof, to contact with a sample,
determining presence or absence or a level of the target molecule in the sample, wherein the first recombinant antibody and the second recombinant antibody are capable of generating a förster resonance energy transfer (FRET) reaction when binding the target molecule, and wherein the target molecule is Staphylococcus aureus Enterotoxin A,

A förster resonance energy transfer (FRET) pair comprising a first recombinant antibody or antigen binding fragment thereof that binds a target molecule and a second recombinant antibody or antigen binding fragment thereof that binds the target molecule at one or more different binding sites compared to the first recombinant antibody or antigen binding fragment thereof, wherein the target molecule is Staphylococcus aureus Enterotoxin A.

A recombinant antibody or antigen binding fragment thereof that binds Staphylococcus aureus Enterotoxin A, wherein the antibody or antigen binding fragment thereof comprises amino acids of one or more complementarity determining regions (CDRs) selected from the group consisting of

a light chain region 1 comprising amino acids 24-35 of SEQ ID NO:
2,
a light chain region 2 comprising amino acids 50-56 of SEQ ID NO:
2,
a light chain region 3 comprising amino acids 90-98 of SEQ ID NO:
2,
a heavy chain region 1 comprising amino acids 30-35 of SEQ ID NO:
1,
a heavy chain region 2 comprising amino acids 50-69 of SEQ ID NO:
1, and
a heavy chain region 3 comprising amino acids 99-108 of SEQ ID NO:
1,
or the antigen binding fragment thereof comprises amino acids of one or more complementarity determining regions (CDRs) selected from the group consisting of
a light chain region 1 comprising amino acids 24-34 of SEQ ID NO:
4,
a light chain region 2 comprising amino acids 50-56 of SEQ ID NO:
4,
a light chain region 3 comprising amino acids 89-97 of SEQ ID NO:
4,
a heavy chain region 1 comprising amino acids 30-35 of SEQ ID NO:
3,
a heavy chain region 2 comprising amino acids 50-69 of SEQ ID NO:
3, and
a heavy chain region 3 comprising amino acids 99-107 of SEQ ID NO:
3.

22 more elements

Dependencies are inferred from the claim text.

Methods, antibodies or antigen binding fragments and other tools for determining target molecules

Life of this patent

Methods, antibodies or antigen binding fragments and other tools for determining target molecules

Life of this patent

Abstract

Claims37