Methods, Compositions and Systems for Respiratory Virus Detection
OwnerAyla Harris, Brian Kruegerinventors
Life of this patent
Estimating the term…
Abstract
Disclosed are methods, compositions and systems to measure respiratory viruses including, but not limited to, SARS-CoV-2, influenza A (Flu A), influenza B (Flu B), or Respiratory Syncytial Virus (RSV). The methods to determine whether a sample from a subject contains an infections respiratory virus may include the steps of obtaining a sample from a subject; performing RT-PCR using primers specific for at least two distinct respiratory viruses; and determining the amount and/or presence of at least one of the at least two distinct respiratory viruses in the sample. In certain cases, the methods and systems comprise multiplex real-time RT-PCR.
Claims20
A method to determine whether a sample from a subject contains an infections respiratory virus comprising:
- obtaining the sample from a subject;
- isolating RNA from the sample;
- performing reverse transcriptase polymerase chain reaction (RT-PCR) amplification using primers and probes specific for the at least two distinct respiratory viruses;
- and
- determining the amount and/or presence of at least one of the at least two distinct respiratory viruses in the sample.
A system to determine whether a sample from a subject contains an infections respiratory virus comprising:
- a station and/or component for isolating RNA from a sample from the subject;
- a station and/or component for performing reverse transcriptase polymerase chain reaction (RT-PCR) amplification using primers and probes specific for at least two distinct respiratory viruses;
- and
- a station and/or component for determining the amount and/or presence of at least one of the at least two distinct respiratory viruses in the sample.
A composition to determine whether a sample from a subject contains an at least one respiratory virus comprising primers and probes comprising at least one of SEQ ID NOs:
- 1-17.
Dependencies are inferred from the claim text.