Hilum color alleles in soybean
AssigneeSyngenta Crop Protection AG
InventorsJose Aponte-Rivera, Azhaguvel Perumal, Dominic Michael Tucker
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Abstract
The present disclosure relates to compositions and methods for identifying, selecting and/or producing soybean plants having particular desired hilum colors. In particular, identifying, selecting, and/or producing soybean plants having hilum colors of yellow or imperfect yellow
Claims20
A method of detecting a soybean plant comprising a genotype associated with a yellow/imperfect yellow hilum color phenotype by:
- a. obtaining a DNA or RNA sample from a tissue of at least one soybean plant;
- b. determining the combined allelic state of the molecular markers represented by
- i. Tat position 164 of SEQ ID NO:
- 1;
- ii. either A at position 251 as in SEQ ID NO:
- 2 or C at position 251 as in SEQ ID NO:
- 3;
- iii. A at position 201 of SEQ ID NO:
- 4;
- iv. Gat position 61 of SEQ ID NO:
- 5;
- v. G at position 201 of SEQ ID NO:
- 6;
- and
- vi. either A at position 274 as in SEQ ID NO:
- 7 or C at position 274 as in SEQ ID NO:
- 8;
- and
- c. identifying at least one soybean plant in which at least one of the above allelic state combinations are associated with the desired yellow or imperfect yellow hilum color.
A method of increasing the seed lot homogeneity in successive generations of a population of soybean plants, the method comprising the steps of:
- a. crossing two parental soybean plants to generate an F1 population of soybean plants;
- b. self-crossing at least one soybean plant of the F1 population to generate an F2 population of soybean plants;
- c. obtaining a DNA or RNA sample from a tissue of at least one soybean plant of the F2 population;
- and
- d. determining whether the soybean plant comprises the combined allelic state of molecular markers represented by
- i. Tat position 164 of SEQ ID NO:
- 1;
- ii. either A at position 251 as in SEQ ID NO:
- 2 or C at position 251 as in SEQ ID NO:
- 3;
- iii. A at position 201 of SEQ ID NO:
- 4;
- iv. Gat position 61 of SEQ ID NO:
- 5;
- v. G at position 201 of SEQ ID NO:
- 6;
- and
- vi. either A at position 274 as in SEQ ID NO:
- 7 or C at position 274 as in SEQ ID NO:
- 8;
- e. selecting at least one plant of the F2 based on the allelic state determined in step (d) and self-crossing the selected plant to generate an F3 population of soybean plants;
- thus increasing the seed lot homogeneity of successive generations.
A composition comprising one or more amplification primer pairs capable of initiating DNA polymerization by a DNA polymerase on a Glycine max nucleic acid template to generate a Glycine max marker amplicon wherein the Glycine max amplicon can be used to identify the Glycine max marker comprising a nucleotide sequence of any of SEQ ID NOS:
- 1-8.
Dependencies are inferred from the claim text.